By: Avan Lal, Meghana Yerraguntla, and Krishna Gunti
After synthesizing silver nanoparticles (AgNPs) with sodium citrate as a reducing agent, an extensive examination of their biological activity is conducted. The antibacterial ability of AgNPs’ was evaluated using microbial cultures, and the outcomes demonstrated that they significantly suppressed the growth of bacteria. The interactions between AgNPs and biological molecules are then clarified by biochemical experiments, which emphasize the modification of enzyme function and affinity for proteins and nucleic acids.
Additionally, alamarBlue dye is used in cell viability tests to assess the cytotoxic effects of AgNPs, revealing dose-dependent effects on mammalian cell lines. A reactive oxygen species (ROS) assay provided more about AgNPs’ capacity to cause oxidative stress reduction in treated cells, offering understanding into the mechanisms behind their cytotoxicity as an anti-inflammatory agent. By using DCFDA dye, we can assay the relative level of ROS. The reduction of DCFDA dye by ROS indicate the level of ROS in a particular cell line.
The biological consequences of AgNPs, including their antibacterial activity, biochemical interactions, and cellular responses, are thoroughly understood thanks to this work. These results highlight the significance of evaluating AgNPs’ safety and effectiveness in biological systems while also advancing the investigation of AgNPs for a range of biomedical and environmental application. Conducting studies on LPS induced inflammation in the K562 cell line and AgNps inhibitory activity on ROS may generate profound insights into therapy for inflammatory diseases such as rheumatoid Arthritis.
Keywords: Nanoparticle,Biochemical,Anti-Inflammatory,ROS,Mammalian cell
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