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By: Hariprasad M. Paikrao, Archana Mahakalkar, Tisha Ramteke, and Sakshi Fulke.
1. Archana Mahakalkar, Assistant Professor, Department of Zoology, Institute of Science, Nagpur, Maharashtra, India.
2. Hariprasad M. Paikrao, Assistant Professor, Department of Biology, Government Institute
of Forensic Science, Nagpur, Maharashtra, India.
3. Tisha Ramteke, UG Student, Government Institute of Forensic Science Nagpur, Maharashtra, India.
4. Sakshi Fulke, UG Student, Government Institute of Forensic Science Nagpur, Maharashtra, India.
Green synthesis of nanoparticles is a developing technology known for its safe and environmentally
friendly approach. Fungi possess essential features in fabricating biogenic nanoparticles of different
metals due to their enzymatic capabilities. Myconanoparticles derived from Fusarium species are
synthesized in multiple stages. The most utilized species for nanoparticle creation are Fusarium
oxysporum and Fusarium solani. This study aims to investigate the growth and conduct microscopic
inspection of Fusarium sp. The extraction was achieved endogenously and exogenously, and later the
extract was utilized for the synthesis of silver nanoparticles. The enzymatic activities of Fusarium
extracts, such as reductase and oxidoreductase, play a significant role in fungal-mediated biogenic
synthesis of silver nanoparticles. The synthesis was optimized and subsequently, the nanoparticle
characterization was carried out using UV-visible spectroscopy and Fourier-transform infrared
spectroscopy. The present paper discusses the significance of silver nanoparticles over other metal
nanoparticles, such as zinc, titanium oxide, platinum, and magnetite, in terms of stability, shape, size,
and biological activities. Biogenic nanoparticles can be employed for several applications such as in
forensic science, agriculture, and pharmaceuticals. The synthesized nanoparticles are useful for the
development of nano-biosensors to detect bodily fluids, such as semen, saliva, sweat, etc., from the
crime scene in forensic investigations, along with onsite detection of drugs of abuse. Similarly, the
applications of silver nanoparticles include fabricating diagnostic biosensors for non-invasive
detection of marker analytes in various diseases such as cancer. The nanoparticle-mediated drug
delivery can be utilized using fungal-mediated silver nanoparticles and will have the advantage of
biological compatibility over chemically synthesized nanoparticles.
Keywords: Nanotechnology, forensic, myco-nanoparticles, fungi, Fusarium oxysporum.
Citation:
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