The growing interest in lipase production is driven by the potential biotechnological applications. In present study lipase producing enzyme using strain Candida tropicalis isolates by using tributyrin agar plate method and confirmation on sprit blue agar which showed the positive result via a zone of hydrolysis. lipase enzyme was carried out by SSF and SMF showing more effective production SSF (25.97 ± 0.47 U/ml/min) as compared to SMF (10.27 ± 0.11 U/ml/min). In SSF, wheat bran used as carbon source and 2% olive oil act as a substrate to give the maximum activity of lipase & optimization was carried out by maximum activity at 30oC at the 9 pH for 1-hour incubation time. The crude lipase activity estimated (55.5 U/ml/min) and partial purification using ammonium sulphate followed by dialysis, which increased the activity in 80% saturation (130 U/ml/min) removing of contaminating proteins and other impurities. The enzyme substrate kinetics analysis using Lineweaver–Burk plot, the values of km and Vmax derived 2.55% and 37.73 (U/ml/min), respectively, indicates the catalytic efficiency of purified lipase towards substrate hydrolysis. The potential application of lipase in bio scouring was investigated enzymatic removal of impurities from cotton fabric and improved hydrophilicity for wet process. Various parameters – such as pH, temperature, time, enzyme concentration – were optimized to remove the fatty acid and other impurity. The enzymes were also successfully applied as a biocatalyst for biodiesel production from waste cooking oil. Lipase is a biocatalyst that hydrolyzes triglycerides (fats) into fatty acid and glycerol. Waste cooking oil is good source of producing biodiesel with esterification & methyl acetate act as an acyl acceptor. Yield was standardized by optimizing various parameters. biodiesel give 70% conversion yield in 40oC for 24 hours, 1:3 molar: ratio with 6% of concentration of free lipase enzyme. Qualitative analysis & primary confirmation test done by thin layer chromatography with 0.60 RF value of alkyl ester.the present study was found eco-friendly biocatalyst with significant potential for sustainable textile and various industry.

Keywords: Candida tropicalis, lipase production, optimization SSF fermentation, km & Vmax graph, bio scouring, cotton fabric, waste cooking oil, biodiesel, trans esterification

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