Chromatography (Paper and Thin-layer chromatography) Techniques and Applications

Purendra Singh

International Journal of Analytical and Applied Chemistry

Chromatography (Paper and Thin-layer chromatography) Techniques and Applications

Purendra Singh

Abstract

Chromatography technique for separation of the component and a mixture on the basis of relative amount of each solute distribution between a moving fluid stream, called mobile phase and stationary phase. Chromatography is an essential biophysical technique that allows the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Proteins can be purified based on features such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with the stationary phase. Four separation methods based on molecular traits and interaction type use mechanisms of ion exchange, surface adsorption, partition and size exclusion. Other chromatography processes are based on the stationary bed, including column, thin layer, and paper chromatography. Column chromatography is one of the most familiar methods of protein purification.Keywords: Chromatography, Paper chromatography, Thin-layer chromatography, Applications

Full text

The full text of this article is available to subscribers.

On a subscribing campus you are let in by network address, with no login at all.

One-off purchase. Permanent access to this article, no account needed.

A year of online access to every article in International Journal of Analytical and Applied Chemistry. Or choose several journals →

Article details

Chromatography (Paper and Thin-layer chromatography) Techniques and Applications

Abstract

Read more of it

Publish your article

Abstract

Related articles

Cryogenic Electron Microscopy Reveals Conformational Dynamics and Catalytic Mechanisms of Human Fatty Acid Synthase in De Novo Lipid Biosynthesis

General and Organic Chemistry Laboratory Experiments for Science, Pre-medical, and Pharmacy Students – Chapter V: Organic Synthesis

Photo-Catalytic Degradation of Dyes Using Graphene Oxide–Dithizone Composite

Spatial Variation and Pollution Assessment of Selected Water Quality Variables in the Luubara Creek, Niger Delta, Nigeria

Chemistry of Mehandi (Henna): Natural Dyeing Mechanism, Composition, and Applications

Agro Seed Oil Production through Mechanothermal Extraction: Exploring its Potential for Chemical, Pharmaceutical, and Food Industries

Read more of it

Publish your article