Sorghum vulgare, a gramineae of commercial value, requires NH₄NO₃ for healthy growth, that in excess causes loss of soil productivity and the release of N₂O, a greenhouse gas that contributes to global warming. An alternative solution to reduce and optimize NH₄NO₃ with: Azospirillum lipoferum and Rhizobium tropici. The objective of this work was: to analyze the effect of A. lipoferum and R. tropici 50%. For this, A. lipoferum and R. tropici were inoculated in S. vulgare in a hydroponic system in Leonard jars, under a randomized block experimental design with 5 treatments and 5 repetitions each. The response variables were percentage (%) of germination, aerial and root phenology; plant height and root length, biomass: aerial fresh weight and radical fresh weight, experimental data were analyzed by Tukey. The results showed 100% germination of S. vulgare with A. lipoferum and R. tropici statistically different numerical value compared to the 75% S. vulgare used as relative control (RC) with 100% NH₄NO₃ not inoculated. S. vulgare to seedling aerial dry weight (ADW) with A. lipoferum and R. tropici was 0.90g statistically different numerical value compared to 0.35g ADW of S. vulgare used as RC. At the flowering level, S. vulgare with A. lipoferum and R. tropici reached 3.17g of radical dry weight (RDW), a statistically different numerical value compared to the 1.59g of RDW of S. vulgare with 100% NH₄NO₃ not inoculated. It was evident that the integration of the metabolic capacity of A. lipoferum combined with the analogue of R. tropici, generated sufficient phytohormones to increase the capacity of S. vulgare for maximum uptake of NH₄NO₃, but non compromising its healthy growth, that avoiding lost soil fertility, contamination of surface water and aquifers, especially the release of N₂O greenhouse gas partly responsible for global warming. Concludes that A. lipoferum and R. tropici in S. vulgar, through a synergistic action, optimized NH₄NO to 50%.

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