Kinetic parameters of crude oil degradation as related to total petroleum hydrocarbon (TPH) were evaluated. The experiment was set-up using crude oil, loamy soil polluted and addition of bio-stimulant (nutrient based substance). The depletion of the pollutant was monitored within the contact time of 7 to 35 days. At intervals of 7 days samples were collected and analyzed to determine the TPH concentration at each bioreactor set-up, through subjecting the samples into Gas Chromatography (GC) analyses. The obtain data were subjected into mathematical evaluation through establishing the relationship of substrate (TPH) degradation concentration with contact time for the purpose of extrapolating the specific rate of TPH degradation (velocity). A plot of the relationship of the specific rate of TPH degradation and TPH concentration degradation of its reciprocal form was achieved. This relationship is known as the LineWeaver Burk Plot which was derived from the Michaelis Menten Model. The relationship enhanced the determination of the kinetic parameters in the area of maximum specific rate of TPH degradation (R_max) the equilibrium constant of TPH degradation (K_TPH). The kinetic parameters are dependent of microbial growth and substrate utilization in each bioreactor, especially at progressive phase. The kinetic parameters are higher in the bioreactors that received the room dried thand sun-dried samples and less in control some of the kinetic parameters were not established because the adopted concept does not obey the Michalis Menten. However, the application of regression methods of plotting will demonstrate the equation of the linear line where the slope and intercept values can be equated to the established concept of

 slope and

 = intercept can be integrated for the purpose of determining the kinetic parameters. The investigation has shown the kinetic parameters values of the mint leaf (Mentha piperita) application in crude oil remediation using 50 g and 100 g dosage in loamy soil of 2 kg and 100 ml of crude oil used as pollutant

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1. McGill WB. Soil restoration following oil spills-a review. J Can Pet Technol. 1977 Apr 1;16(02).
2. Huang Y, Pan H, Wang Q, Ge Y, Liu W, Christie P. Enrichment of the soil microbial community in the bioremediation of a petroleum-contaminated soil amended with rice straw or sawdust. Chemosphere. 2019;224:265–271.
3. Jude K, Durán N, Esposito E. Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil. J Can Pet Technol. 2022;16(2). doi: 2118/77-02-07. ISSN 0021-9487.
4. Kost D, Dick WA, Ye X, Li W, Chen. Bioremediation of hydrocarbon degradation in a petroleum contaminated soil and microbial population and activity determination. Chemosphere Environ Toxicol Risk Assess. 2017.
5. Lawal OM, Nwokem NC. Removal of oil from crude oil polluted water using mango seed bark as sorbent in a packed column. Fed Univ Wukari Trends Sci Technol J. 2017;2(2):973–975.
6. Lee KW, Shim WJ, Kwon OY, Kang J. Size-dependent effects of micro polystyrene particles in the marine copepod Tigriopus japonicus. Environ Sci Technol. 2013;47(19):11278–11283. doi: 1021/es401932b. PMID:23988225. ISSN 0013-936X.
7. Kanwal M, Ullah H, Gulzar A, Sadiq T, Ullah M, et al. Biodegradation of petroleum hydrocarbons and the factors effecting rate of biodegradation. Am J Biomed Sci Res. 2022;16(1). doi: 34297/AJBSR.2022.16.002182.
8. Montagnolli RN, Lopes PRM, Bidoia ED. Screening the toxicity and biodegradability of petroleum hydrocarbons by a rapid colorimetric method. Arch Environ Contam Toxicol. 2015;68(2):342–353. doi: 1007/s00244-014-0112-9. PMID:25537922. S2CID:5249816.
9. Ojewumi ME, Anenih EV, Taiwo OS, Adekeye BT, Awolu OO, Ojewumi EO. A bioremediation study of raw and treated crude petroleum oil polluted soil with Aspergillus niger and Pseudomonas aeruginosa. J Ecol Eng. 2018;19(2):226–235.
10. Ukpaka CP, Sinee BL, Harmony N. Crude oil degradation in loamy soil using neem root extracts: An experimental study. Chem Int. 2020;6(3):160–170.
11. Varjani SJ. Microbial degradation of petroleum hydrocarbons. Bioresour Technol. 2017 Jan 1;223:277–286.
12. Von Moos N, Burkhardt-Holm P, Köhler A. Uptake and effects of microplastics on cells and tissue of the blue mussel Mytilus edulis L. after an experimental exposure. Environ Sci Technol. 2012;46(20):11327–11335. doi: 1021/es302332w. PMID:22963286.
13. Wu M, Wu J, Zhang X, Ye X. Effect of bioaugmentation and biostimulation on hydrocarbon degradation and microbial community composition in petroleum-contaminated loessal soil. Chemosphere. 2019;237:124456.
14. Yang Z, Verpoort F, Dong C, Chen C, Chen S, Kao C. Remediation of petroleum-hydrocarbon contaminated groundwater using optimized in situ chemical oxidation system: batch and column studies. Process Saf Environ Prot. 2020;138:18–26.

Kinetic parameters of crude oil degradation as related to total petroleum hydrocarbon (TPH) were evaluated. The experiment was set-up using crude oil, loamy soil polluted and addition of bio-stimulant (nutrient based substance). The depletion of the pollutant was monitored within the contact time of 7 to 35 days. At intervals of 7 days samples were collected and analyzed to determine the TPH concentration at each bioreactor set-up, through subjecting the samples into Gas Chromatography (GC) analyses. The obtain data were subjected into mathematical evaluation through establishing the relationship of substrate (TPH) degradation concentration with contact time for the purpose of extrapolating the specific rate of TPH degradation (velocity). A plot of the relationship of the specific rate of TPH degradation and TPH concentration degradation of its reciprocal form was achieved. This relationship is known as the LineWeaver Burk Plot which was derived from the Michaelis Menten Model. The relationship enhanced the determination of the kinetic parameters in the area of maximum specific rate of TPH degradation (R_max) the equilibrium constant of TPH degradation (K_TPH). The kinetic parameters are dependent of microbial growth and substrate utilization in each bioreactor, especially at progressive phase. The kinetic parameters are higher in the bioreactors that received the room dried thand sun-dried samples and less in control some of the kinetic parameters were not established because the adopted concept does not obey the Michalis Menten. However, the application of regression methods of plotting will demonstrate the equation of the linear line where the slope and intercept values can be equated to the established concept of

 slope and

 = intercept can be integrated for the purpose of determining the kinetic parameters. The investigation has shown the kinetic parameters values of the mint leaf (Mentha piperita) application in crude oil remediation using 50 g and 100 g dosage in loamy soil of 2 kg and 100 ml of crude oil used as pollutant

🔒 This is a subscription article

Full text is available to subscribers and institutional members. Please choose an option below to access it.

1. McGill WB. Soil restoration following oil spills-a review. J Can Pet Technol. 1977 Apr 1;16(02).
2. Huang Y, Pan H, Wang Q, Ge Y, Liu W, Christie P. Enrichment of the soil microbial community in the bioremediation of a petroleum-contaminated soil amended with rice straw or sawdust. Chemosphere. 2019;224:265–271.
3. Jude K, Durán N, Esposito E. Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil. J Can Pet Technol. 2022;16(2). doi: 2118/77-02-07. ISSN 0021-9487.
4. Kost D, Dick WA, Ye X, Li W, Chen. Bioremediation of hydrocarbon degradation in a petroleum contaminated soil and microbial population and activity determination. Chemosphere Environ Toxicol Risk Assess. 2017.
5. Lawal OM, Nwokem NC. Removal of oil from crude oil polluted water using mango seed bark as sorbent in a packed column. Fed Univ Wukari Trends Sci Technol J. 2017;2(2):973–975.
6. Lee KW, Shim WJ, Kwon OY, Kang J. Size-dependent effects of micro polystyrene particles in the marine copepod Tigriopus japonicus. Environ Sci Technol. 2013;47(19):11278–11283. doi: 1021/es401932b. PMID:23988225. ISSN 0013-936X.
7. Kanwal M, Ullah H, Gulzar A, Sadiq T, Ullah M, et al. Biodegradation of petroleum hydrocarbons and the factors effecting rate of biodegradation. Am J Biomed Sci Res. 2022;16(1). doi: 34297/AJBSR.2022.16.002182.
8. Montagnolli RN, Lopes PRM, Bidoia ED. Screening the toxicity and biodegradability of petroleum hydrocarbons by a rapid colorimetric method. Arch Environ Contam Toxicol. 2015;68(2):342–353. doi: 1007/s00244-014-0112-9. PMID:25537922. S2CID:5249816.
9. Ojewumi ME, Anenih EV, Taiwo OS, Adekeye BT, Awolu OO, Ojewumi EO. A bioremediation study of raw and treated crude petroleum oil polluted soil with Aspergillus niger and Pseudomonas aeruginosa. J Ecol Eng. 2018;19(2):226–235.
10. Ukpaka CP, Sinee BL, Harmony N. Crude oil degradation in loamy soil using neem root extracts: An experimental study. Chem Int. 2020;6(3):160–170.
11. Varjani SJ. Microbial degradation of petroleum hydrocarbons. Bioresour Technol. 2017 Jan 1;223:277–286.
12. Von Moos N, Burkhardt-Holm P, Köhler A. Uptake and effects of microplastics on cells and tissue of the blue mussel Mytilus edulis L. after an experimental exposure. Environ Sci Technol. 2012;46(20):11327–11335. doi: 1021/es302332w. PMID:22963286.
13. Wu M, Wu J, Zhang X, Ye X. Effect of bioaugmentation and biostimulation on hydrocarbon degradation and microbial community composition in petroleum-contaminated loessal soil. Chemosphere. 2019;237:124456.
14. Yang Z, Verpoort F, Dong C, Chen C, Chen S, Kao C. Remediation of petroleum-hydrocarbon contaminated groundwater using optimized in situ chemical oxidation system: batch and column studies. Process Saf Environ Prot. 2020;138:18–26.