Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae

Dowluru S. V. G. K. Kaladhar; Vinay Kumar; Dowluru V. L. Lolini

Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae

Dowluru S. V. G. K. Kaladhar, Vinay Kumar, Dowluru V. L. Lolini | International Journal of Plant Biotechnology | Vol 12, Issue 02 | pp. 55-61 | ISSN: 2456–0162

Abstract

*Author for CorrespondenceDowluru SVGK Kaladhar E-mail: [email protected] ¹Professor, Department of Microbiology and Bioinformatics, UTD, Atal Bihari Vajpayee University, Bilaspur, Chhattisgarh, India²Student, Department of Microbiology and Bioinformatics, UTD, Atal Bihari Vajpayee University, Bilaspur, Chhattisgarh, India³Student, Department of Biotechnology, DP Vipra College, Bilaspur, Chhattisgarh, India Received Date: June 17, 2026Accepted Date: June 24, 2026Published Date: August 15, 2026 Citation: Dowluru S. V. G. K. Kaladhar, Vinay Kumar, Dowluru V. L. Lolini. Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae. International Journal of Plant Biotechnology. 2026; 12(2): 55–61p.

Abstract

In the present study, about 42% of seed oil was obtained from fresh seeds of Sinapis alba (yellow mustard). The oil was not formed in the zone for gelatin degradation. In the present experimentation, the yellow mustard (Sinapis alba L.) seed oil with Trypsin and Proteinase K has shown gelatin degradation. The oil from yellow mustard (Sinapis alba L.) seed oil also contains Carbohydrate, Protein, Cholesterol, Steroids, Terpenoids and Cardiac Glycosides. The seed oil and Petroleum-ether mix with Saccharificated oil (50:50) has shown little activity with Candida, Enterococcus, Pseudomonas and Staphylococcus. A small zone of antimicrobial activity was observed with Petroleum-ether dissolved with microbial oil (50:50). Sinalbin, Sinapic Acid, Canolol, 2-(4-Hydroxiphenyl)acitonitrile are selected as ligands. Sinalbin has shown good binding activity with microbial proteins showing good antimicrobial activity.

Keywords: Oil extraction, Sinapis alba L. seeds, microbial oil, molecular docking, Sinalbin antimicrobial activity

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References

Forrest RD. Early history of wound treatment. J R Soc Med. 1982;75(3):198–205.
Rasool A, Bhat KM, Sheikh AA, Jan A, Hassan S. Medicinal plants: Role, distribution and future. J Pharmacogn Phytochem. 2020;9(2):2111–4.
Lysak MA, Koch MA. Phylogeny, genome, and karyotype evolution of crucifers (Brassicaceae). In: Genetics and Genomics of the Brassicaceae. 2011. p. 1–31.
Lietzow J. Biologically active compounds in mustard seeds: A toxicological perspective. Foods. 2021;10(9):2089.
Pratap A, Gupta SK. Biology and ecology of wild crucifers. In: Biology and Breeding of Crucifers. 2009. p. 37–67.
Ekanayake A, Strife RJ, Zehentbauer GN, David JR. Yellow or white mustard (Sinapis alba L.) oils. In: Essential Oils in Food Preservation, Flavor and Safety. Academic Press; 2016. p. 857–63.
Sawicka B, Kotiuk E, Kiełtyka-Dadasiewicz A, Krochmal-Marczak B. Fatty acids composition of mustard oil from two cultivars and physico-chemical characteristics of the seeds. J Oleo Sci. 2020;69(3):207–17.
Mitrović PM, Stamenković OS, Banković-Ilić I, Djalović IG, Nježić ZB, Farooq M, et al. White mustard (Sinapis alba L.) oil in biodiesel production: A review. Front Plant Sci. 2020;11:299.
Abiodun OA. The role of oilseed crops in human diet and industrial use. In: Oilseed Crops: Yield and Adaptations Under Environmental Stress. 2017. p. 249–63.
Popova IE, Morra MJ. Simultaneous quantification of sinigrin, sinalbin, and anionic glucosinolate hydrolysis products in Brassica juncea and Sinapis alba seed extracts using ion chromatography. J Agric Food Chem. 2014;62(44):10687–93.
Thiyam U, Stöckmann H, ZumFelde T, Schwarz K. Antioxidative effect of the main sinapic acid derivatives from rapeseed and mustard oil by-products. Eur J Lipid Sci Technol. 2006;108(3):239–48.
Martinović N, Polak T, Ulrih NP, Abramovič H. Mustard seed: Phenolic composition and effects on lipid oxidation in oil, oil-in-water emulsion and oleogel. Ind Crops Prod. 2020;156:112851.
Thiyam-Holländer U, Aladedunye F, Logan A, Yang H, Diehl BW. Identification and quantification of canolol and related sinapate precursors in Indian mustard oils and Canadian mustard products. Eur J Lipid Sci Technol. 2014;116(12):1664–74.
Morra MJ, Popova IE, Boydston RA. Bioherbicidal activity of Sinapis alba seed meal extracts. Ind Crops Prod. 2018;115:174–81.
Kunitz M. Crystalline soybean trypsin inhibitor II. General properties. J Gen Physiol. 1947;30(4):291–310.
Kaladhar DS, Kenar SK, Meesala S, Sahu S. Biofuel production, phytochemical analysis and in silico analysis for active compound valorization from Jatropha gossypifolia seeds. J Pharm Negat Results. 2023:1555–65.
Grygier A. Mustard seeds as a bioactive component of food. Food Rev Int. 2022:1–14.

How to cite this article

APA

Kaladhar, D. S. V. G. K., Kumar, V., & Lolini, D. V. L. (2026). Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae. International Journal of Plant Biotechnology, 12(02), 55-61.

MLA

Kaladhar, Dowluru S. V. G. K., et al. “Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae.” International Journal of Plant Biotechnology, vol. 12, no. 02, 2026, pp. 55-61.

Chicago

Dowluru S. V. G. K. Kaladhar, Vinay Kumar, and Dowluru V. L. Lolini. “Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae.” International Journal of Plant Biotechnology 12, no. 02 (2026): 55-61.

Vancouver

Kaladhar DSVGK, Kumar V, Lolini DVL. Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae. International Journal of Plant Biotechnology. 2026;12(02):55-61.

BibTeX

@article{KaladharDSVGK2026,
author = {Dowluru S. V. G. K. Kaladhar and Vinay Kumar and Dowluru V. L. Lolini},
title = {Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae},
journal = {International Journal of Plant Biotechnology},
year = {2026},
volume = {12},
number = {02},
pages = {55--61},
issn = {2456–0162},
url = {https://journalspub.com/publication/ijpb/article=27497}
}

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Dowluru S. V. G. K. Kaladhar, Vinay Kumar, Dowluru V. L. Lolini | International Journal of Plant Biotechnology | Vol 12, Issue 02 | pp. 55-61 | ISSN: 2456–0162

Abstract

Abstract

Keywords: Oil extraction, Sinapis alba L. seeds, microbial oil, molecular docking, Sinalbin antimicrobial activity

🔒 This is a subscription article

Full text is available to subscribers and institutional members. Please choose an option below to access it.

Subscribe Purchase this article Institutional / Login access

References

Forrest RD. Early history of wound treatment. J R Soc Med. 1982;75(3):198–205.
Rasool A, Bhat KM, Sheikh AA, Jan A, Hassan S. Medicinal plants: Role, distribution and future. J Pharmacogn Phytochem. 2020;9(2):2111–4.
Lysak MA, Koch MA. Phylogeny, genome, and karyotype evolution of crucifers (Brassicaceae). In: Genetics and Genomics of the Brassicaceae. 2011. p. 1–31.
Lietzow J. Biologically active compounds in mustard seeds: A toxicological perspective. Foods. 2021;10(9):2089.
Pratap A, Gupta SK. Biology and ecology of wild crucifers. In: Biology and Breeding of Crucifers. 2009. p. 37–67.
Ekanayake A, Strife RJ, Zehentbauer GN, David JR. Yellow or white mustard (Sinapis alba L.) oils. In: Essential Oils in Food Preservation, Flavor and Safety. Academic Press; 2016. p. 857–63.
Sawicka B, Kotiuk E, Kiełtyka-Dadasiewicz A, Krochmal-Marczak B. Fatty acids composition of mustard oil from two cultivars and physico-chemical characteristics of the seeds. J Oleo Sci. 2020;69(3):207–17.
Mitrović PM, Stamenković OS, Banković-Ilić I, Djalović IG, Nježić ZB, Farooq M, et al. White mustard (Sinapis alba L.) oil in biodiesel production: A review. Front Plant Sci. 2020;11:299.
Abiodun OA. The role of oilseed crops in human diet and industrial use. In: Oilseed Crops: Yield and Adaptations Under Environmental Stress. 2017. p. 249–63.
Popova IE, Morra MJ. Simultaneous quantification of sinigrin, sinalbin, and anionic glucosinolate hydrolysis products in Brassica juncea and Sinapis alba seed extracts using ion chromatography. J Agric Food Chem. 2014;62(44):10687–93.
Thiyam U, Stöckmann H, ZumFelde T, Schwarz K. Antioxidative effect of the main sinapic acid derivatives from rapeseed and mustard oil by-products. Eur J Lipid Sci Technol. 2006;108(3):239–48.
Martinović N, Polak T, Ulrih NP, Abramovič H. Mustard seed: Phenolic composition and effects on lipid oxidation in oil, oil-in-water emulsion and oleogel. Ind Crops Prod. 2020;156:112851.
Thiyam-Holländer U, Aladedunye F, Logan A, Yang H, Diehl BW. Identification and quantification of canolol and related sinapate precursors in Indian mustard oils and Canadian mustard products. Eur J Lipid Sci Technol. 2014;116(12):1664–74.
Morra MJ, Popova IE, Boydston RA. Bioherbicidal activity of Sinapis alba seed meal extracts. Ind Crops Prod. 2018;115:174–81.
Kunitz M. Crystalline soybean trypsin inhibitor II. General properties. J Gen Physiol. 1947;30(4):291–310.
Kaladhar DS, Kenar SK, Meesala S, Sahu S. Biofuel production, phytochemical analysis and in silico analysis for active compound valorization from Jatropha gossypifolia seeds. J Pharm Negat Results. 2023:1555–65.
Grygier A. Mustard seeds as a bioactive component of food. Food Rev Int. 2022:1–14.

How to cite this article

APA

MLA

Chicago

Vancouver

BibTeX

@article{KaladharDSVGK2026,
author = {Dowluru S. V. G. K. Kaladhar and Vinay Kumar and Dowluru V. L. Lolini},
title = {Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae},
journal = {International Journal of Plant Biotechnology},
year = {2026},
volume = {12},
number = {02},
pages = {55--61},
issn = {2456–0162},
url = {https://journalspub.com/publication/ijpb/article=27497}
}

Dowluru S. V. G. K. Kaladhar, Vinay Kumar, Dowluru V. L. Lolini | International Journal of Plant Biotechnology | Vol 12, Issue 02 | pp. 55-61 | ISSN: 2456–0162

Abstract

Abstract

Keywords: Oil extraction, Sinapis alba L. seeds, microbial oil, molecular docking, Sinalbin antimicrobial activity

🔒 This is a subscription article

Full text is available to subscribers and institutional members. Please choose an option below to access it.

Subscribe Purchase this article Institutional / Login access

References

Forrest RD. Early history of wound treatment. J R Soc Med. 1982;75(3):198–205.
Rasool A, Bhat KM, Sheikh AA, Jan A, Hassan S. Medicinal plants: Role, distribution and future. J Pharmacogn Phytochem. 2020;9(2):2111–4.
Lysak MA, Koch MA. Phylogeny, genome, and karyotype evolution of crucifers (Brassicaceae). In: Genetics and Genomics of the Brassicaceae. 2011. p. 1–31.
Lietzow J. Biologically active compounds in mustard seeds: A toxicological perspective. Foods. 2021;10(9):2089.
Pratap A, Gupta SK. Biology and ecology of wild crucifers. In: Biology and Breeding of Crucifers. 2009. p. 37–67.
Ekanayake A, Strife RJ, Zehentbauer GN, David JR. Yellow or white mustard (Sinapis alba L.) oils. In: Essential Oils in Food Preservation, Flavor and Safety. Academic Press; 2016. p. 857–63.
Sawicka B, Kotiuk E, Kiełtyka-Dadasiewicz A, Krochmal-Marczak B. Fatty acids composition of mustard oil from two cultivars and physico-chemical characteristics of the seeds. J Oleo Sci. 2020;69(3):207–17.
Mitrović PM, Stamenković OS, Banković-Ilić I, Djalović IG, Nježić ZB, Farooq M, et al. White mustard (Sinapis alba L.) oil in biodiesel production: A review. Front Plant Sci. 2020;11:299.
Abiodun OA. The role of oilseed crops in human diet and industrial use. In: Oilseed Crops: Yield and Adaptations Under Environmental Stress. 2017. p. 249–63.
Popova IE, Morra MJ. Simultaneous quantification of sinigrin, sinalbin, and anionic glucosinolate hydrolysis products in Brassica juncea and Sinapis alba seed extracts using ion chromatography. J Agric Food Chem. 2014;62(44):10687–93.
Thiyam U, Stöckmann H, ZumFelde T, Schwarz K. Antioxidative effect of the main sinapic acid derivatives from rapeseed and mustard oil by-products. Eur J Lipid Sci Technol. 2006;108(3):239–48.
Martinović N, Polak T, Ulrih NP, Abramovič H. Mustard seed: Phenolic composition and effects on lipid oxidation in oil, oil-in-water emulsion and oleogel. Ind Crops Prod. 2020;156:112851.
Thiyam-Holländer U, Aladedunye F, Logan A, Yang H, Diehl BW. Identification and quantification of canolol and related sinapate precursors in Indian mustard oils and Canadian mustard products. Eur J Lipid Sci Technol. 2014;116(12):1664–74.
Morra MJ, Popova IE, Boydston RA. Bioherbicidal activity of Sinapis alba seed meal extracts. Ind Crops Prod. 2018;115:174–81.
Kunitz M. Crystalline soybean trypsin inhibitor II. General properties. J Gen Physiol. 1947;30(4):291–310.
Kaladhar DS, Kenar SK, Meesala S, Sahu S. Biofuel production, phytochemical analysis and in silico analysis for active compound valorization from Jatropha gossypifolia seeds. J Pharm Negat Results. 2023:1555–65.
Grygier A. Mustard seeds as a bioactive component of food. Food Rev Int. 2022:1–14.

How to cite this article

APA

MLA

Chicago

Vancouver

BibTeX

@article{KaladharDSVGK2026,
author = {Dowluru S. V. G. K. Kaladhar and Vinay Kumar and Dowluru V. L. Lolini},
title = {Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae},
journal = {International Journal of Plant Biotechnology},
year = {2026},
volume = {12},
number = {02},
pages = {55--61},
issn = {2456–0162},
url = {https://journalspub.com/publication/ijpb/article=27497}
}