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By: Sakshi Chaudhary, Neetu Saharan, and Bibhas K Bhunia
1Department of Biotechnology, Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar, Ghaziabad, India.
2Department of Microbiology, Parul Institute of Applied Sciences (PIAS), Parul University, Vadodara, Gujarat- 391760, India
Recent advancements have enabled the application of three-dimensional (3D) printing technologies to biocompatible materials, cells, and supporting components, leading to the emergence of 3D bioprinting. Surgical limitations necessitate alternative methods for repairing and replacing diseased and damaged tissue. Regenerative medicine, a rapidly expanding field of research, has already seen the successful application of engineered tissues in patients. However, the demand for these tissues far exceeds the available supply, highlighting the need for a faster and more accurate production method. The 3D bioprinting shows significant potential for artificial organ printing and regenerative medicine. Concurrently, stem cells, including human induced pluripotent stem cells, have revolutionized tissue regeneration and human disease modeling, providing an unlimited cell source for these applications. The capability to reprogram patient-specific cells offers deeper insight into disease mechanisms and phenotypic variability. Various types of stem cells from different lineages and potencies have been successfully used in 3D bioprinting. The methods employed range from micro-extrusion bioprinting, inkjet bioprinting, and laser-assisted bioprinting to newer techniques, such as scaffold-free spheroid-based bioprinting. This review examines the latest advancements, applications, limitations, and prospects of 3D bioprinting with stem cells, categorized by organ systems.
Citation:
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