The present study investigates the acoustic and thermodynamic behavior of aqueous solutions of L-
Histidine across a concentration range of 0.01 m to 0.1 m and temperatures varying from 293.15 K to 318.15 K in 5 K intervals. Ultrasonic velocity (μ), density (ρ), and viscosity (η) measurements were
conducted with high precision to understand the solution behavior under different thermal conditions.
Temperature constancy during measurements was ensured using a refrigerated circulatory bath with
water as the thermal medium, thereby enhancing the reliability of the experimental data. From the
primary measurements, various acoustic and thermodynamic parameters were derived using
established theoretical equations. These include isentropic compressibility (βa), isothermal
compressibility (βi), apparent molar volume (Φv), partial molar volume at infinite dilution (Φv0),
viscosity coefficients A and B (based on Jones–Dole relation), and hydration number (nh). The analysis
reveals complex molecular interactions within the L-Histidine aqueous system, including solute–solvent
and solute–solute interactions. Anomalies or deviations in trends suggest the possible formation of
hydration shells or structured water molecules around the zwitterionic centers of L-Histidine. These
results contribute to a deeper understanding of biomolecular solvation phenomena and the
physicochemical behavior of amino acid solutions under varying thermodynamic conditions. The
insights are especially relevant for biochemical, pharmaceutical, and solution chemistry applications
where knowledge of solute behavior at different temperatures is essential for formulation, stability
studies, and reaction kinetics.

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The present study investigates the acoustic and thermodynamic behavior of aqueous solutions of L-
Histidine across a concentration range of 0.01 m to 0.1 m and temperatures varying from 293.15 K to 318.15 K in 5 K intervals. Ultrasonic velocity (μ), density (ρ), and viscosity (η) measurements were
conducted with high precision to understand the solution behavior under different thermal conditions.
Temperature constancy during measurements was ensured using a refrigerated circulatory bath with
water as the thermal medium, thereby enhancing the reliability of the experimental data. From the
primary measurements, various acoustic and thermodynamic parameters were derived using
established theoretical equations. These include isentropic compressibility (βa), isothermal
compressibility (βi), apparent molar volume (Φv), partial molar volume at infinite dilution (Φv0),
viscosity coefficients A and B (based on Jones–Dole relation), and hydration number (nh). The analysis
reveals complex molecular interactions within the L-Histidine aqueous system, including solute–solvent
and solute–solute interactions. Anomalies or deviations in trends suggest the possible formation of
hydration shells or structured water molecules around the zwitterionic centers of L-Histidine. These
results contribute to a deeper understanding of biomolecular solvation phenomena and the
physicochemical behavior of amino acid solutions under varying thermodynamic conditions. The
insights are especially relevant for biochemical, pharmaceutical, and solution chemistry applications
where knowledge of solute behavior at different temperatures is essential for formulation, stability
studies, and reaction kinetics.

🔒 This is a subscription article

Full text is available to subscribers and institutional members. Please choose an option below to access it.