Hariprasad M. Paikrao, Archana Mahakalkar, Tisha Ramteke, Sakshi Fulke | International Journal of Applied Nanotechnology | Vol 12, Issue 1 | pp. 8-17 | ISSN: 2455-8524
Abstract
Green synthesis of nanoparticles is a developing technology known for its safe and environmentally friendly approach. Fungi possess essential features in fabricating biogenic nanoparticles of different metals due to their enzymatic capabilities. Myconanoparticles derived from Fusarium species are synthesized in multiple stages. The most utilized species for nanoparticle creation are Fusarium oxysporum and Fusarium solani. This study aims to investigate the growth and conduct microscopic inspection of Fusarium sp. The extraction was achieved endogenously and exogenously, and later the extract was utilized for the synthesis of silver nanoparticles. The enzymatic activities of Fusarium extracts, such as reductase and oxidoreductase, play a significant role in fungal-mediated biogenic synthesis of silver nanoparticles. The synthesis was optimized and subsequently, the nanoparticle characterization was carried out using UV-visible spectroscopy and Fourier-transform infrared spectroscopy. The present paper discusses the significance of silver nanoparticles over other metal nanoparticles, such as zinc, titanium oxide, platinum, and magnetite, in terms of stability, shape, size, and biological activities. Biogenic nanoparticles can be employed for several applications such as in forensic science, agriculture, and pharmaceuticals. The synthesized nanoparticles are useful for the development of nano-biosensors to detect bodily fluids, such as semen, saliva, sweat, etc., from the crime scene in forensic investigations, along with onsite detection of drugs of abuse. Similarly, the applications of silver nanoparticles include fabricating diagnostic biosensors for non-invasive detection of marker analytes in various diseases such as cancer. The nanoparticle-mediated drug delivery can be utilized using fungal-mediated silver nanoparticles and will have the advantage of biological compatibility over chemically synthesized nanoparticles
Keywords: Nanotechnology, forensic, myco-nanoparticles, fungi, Fusarium oxysporum.
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1. Taniguchi N. On the basic concept of nanotechnology. In: Proceedings of the Japan Society of Precision Engineering; 1974.
2. Narayanan KB, Sakthivel N. Biological synthesis of metal nanoparticles by microbes. Adv Colloid Interface Sci. 2010;156(1–2):1–13.
3. Maynard A, Michelson E. The nanotechnology consumer products inventory. Washington (DC): Woodrow Wilson International Center for Scholars; 2006.
4. Alvarez-Puebla RA, Aroca RF. Surface-enhanced Raman scattering for ultrasensitive chemical analysis of 1- and 2-naphthalenethiols. Analyst. 2004;129(12):1251–1256.
5. Rai M, Yadav A, Bridge P, Gade A. Myconanotechnology: A new and emerging science. Appl Mycol. 2009:258–267.
6. Meyer V. Genetic engineering of filamentous fungi—Progress, obstacles and future trends. Biotechnol Adv. 2008;26(2):177–185.
7. Dhillon GS, Brar SK, Kaur S, Verma M. Green approach for nanoparticle biosynthesis by fungi: Current trends and applications. Crit Rev Biotechnol. 2012;32(1):49–73.
8. Sastry RK, Rashmi H, Rao NH, Ilyas SM. Integrating nanotechnology into agri-food systems research in India: A conceptual framework. Technol Forecast Soc Change. 2010;77(4):639–648.
9. Khot LR, Sankaran S, Maja JM, Ehsani R, Schuster EW. Applications of nanomaterials in agricultural production and crop protection: A review. Crop Prot. 2012;35:64–70.
10. Bhainsa KC, D’Souza SF. Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus. Colloids Surf B Biointerfaces. 2006;47(2):160–164.
11. Gericke M, Pinches A. Biological synthesis of metal nanoparticles. Hydrometallurgy. 2006;83(1–4):132–140.
12. Nikam S, Shelar KR, Munde MB, Bhosale TY, Bedake PS, Hagawane RM, et al. Fungal-mediated synthesis of silver nanoparticles using Fusarium oxysporum and their antimicrobial evaluation. Int J. 2024;10(2).
13. Rahli F, Chentouf H, Terbeche R, Chougrani S. Biosynthesis of silver nanoparticles using Fusarium oxysporum and their therapeutic applications. J Appl Nat Sci. 2022;14(4).
14. El-Ansary AE, Omran AAA, Mohamed HI, El-Mahdy OM. Green synthesized silver nanoparticles mediated by Fusarium nygamai isolate AJTYC1: Characterization, antioxidant, antimicrobial, anticancer and photocatalytic activities. Environ Sci Pollut Res. 2023;30(45):100477–100499.
15. Djearamane S, Xiu LJ, Wong LS, Rajamani R, Bharathi D, Kayarohanam S, et al. Antifungal properties of zinc oxide nanoparticles on Candida albicans. Coatings. 2022;12(12):1864.
16. Govender Y, Riddin T, Gericke M, Whiteley CG. Bioreduction of platinum salts into nanoparticles: A mechanistic perspective. Biotechnol Lett. 2009;31(1):95–100.
17. Bharde A, Rautaray D, Bansal V, Ahmad A, Sarkar I, Yusuf SM, et al. Extracellular biosynthesis of magnetite using fungi. Small. 2006;2(1):135–141.
18. Vinayagam Y, DR V. Mycosynthesis of TiO₂ nanoparticles using Aspergillus penicillioides for toxic metal removal and photocatalytic applications. J Water Process Eng. 2024;67:106279.
19. Rai M, Bonde S, Golinska P, Trzcińska-Wencel J, Gade A, Abd-Elsalam KA, et al. Fusarium as a novel fungus for the synthesis of nanoparticles: Mechanism and applications. J Fungi. 2021;7(2):139.
20. Fatima F, Wahid I. Eco-friendly synthesis of silver and copper nanoparticles by Schizophyllum commune fungus and its biomedical applications. Int J Environ Sci Technol. 2022;19(8):7915–7926.
21. Husseiny SM, Salah TA, Anter HA. Biosynthesis of size-controlled silver nanoparticles by Fusarium oxysporum, their antibacterial and antitumor activities. Beni Suef Univ J Basic Appl Sci. 2015;4(3):225–231.
22. Durán N, Marcato PD, Durán M, Yadav A, Gade A, Rai M. Mechanistic aspects in the biogenic synthesis of extracellular metal nanoparticles by peptides, bacteria, fungi and plants. Appl Microbiol Biotechnol. 2011;90(5):1609–1624.
23. Sidhu AK, Agrawal SB, Verma N, Kaushal P, Sharma M. Fungal-mediated synthesis of multimetallic nanoparticles: Mechanisms, unique properties and potential applications. Front Nanotechnol. 2025;7:1549713.
24. Santana da Costa T, Delgado GG, Braga CB, Tasic L. Insights into fungal secretomes and their roles in formation and stabilization of biogenic silver nanoparticles. RSC Adv. 2025;15(9):6938–6951.
25. Binupriya AR, Sathishkumar M, Vijayaraghavan K, Yun SI. Bioreduction of trivalent aurum to nano-crystalline gold particles by active and inactive cells and cell-free extract of Aspergillus oryzae var. viridis. J Hazard Mater. 2010;177(1–3):539–545.
26. Jana S, Das D, Bhattacharyya S, Raha S. Mycogenic synthesis of silver nanoparticles using endophytic fungi and their characterization and biological activities with special reference to Fusarium wilt of tomato. Fungal Biol. 2025;129(5):101610.
27. Makarov VV, Love AJ, Sinitsyna OV, Makarova SS, Yaminsky IV, Taliansky ME, et al. “Green” nanotechnologies: Synthesis of metal nanoparticles using plants. Acta Naturae. 2014;6(1):35–44.
28. Sarkar J, Saha S, Chattopadhyay D, Patra S, Acharya K. Mycosynthesis of silver nanoparticles and investigation of their antimicrobial activity. J Nanosci Nanoeng Appl. 2011;1:17–26.
29. Selvi KV, Sivakumar T. Isolation and characterization of silver nanoparticles from Fusarium oxysporum. Int J Curr Microbiol Appl Sci. 2012;1(1):56–62.
30. Prabhu S, Poulose EK. Silver nanoparticles: Mechanism of antimicrobial action, synthesis, medical applications and toxicity effects. Int Nano Lett. 2012;2(1):32.
How to cite this article
@article{PaikraoHM2026,
author = {Hariprasad M. Paikrao and Archana Mahakalkar and Tisha Ramteke and Sakshi Fulke},
title = {Fungal-Mediated Myco-Nanoparticle Synthesis Using Fusarium Species},
journal = {International Journal of Applied Nanotechnology},
year = {2026},
volume = {12},
number = {1},
pages = {8--17},
issn = {2455-8524},
url = {https://journalspub.com/publication/uncategorized/article=24804}
}