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3 articles for “microinjection”
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Recent Trends and Advancements in Transgenesis and Transgenic Technology
Abstract: Transgenic technology is a novel procedure that was introduced in the year 1981. It involves the insertion as well as integration and expression of new/improved genes into the host organism’s genome. This technology has many benefits in animal biotechnology. This article is a brief review on this technology, the ethical issues concerning it as well as its applications in the field of animal biotechnology.
Published in International Journal of Animal Biotechnology and Applications · Vol. 1, Issue 2, 2015 Read article
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Spermmediated gene transfer revisited: optimizing delivery, integration, and germline transmissionin large animal models
Abstract: Spermmediated gene transfer (SMGT) exploits the natural role of spermatozoa as DNA vectors to deliver exogenous genetic material to oocytes, potentially enabling economical production of transgenic large animals without complex embryo micromanipulation (1). Early proofofconcept studies established that mammalian sperm can bind, internalize, and protect exogenous DNA, leading to integration, expression, and germline transmission in various species, including pigs (24). Subsequent mechanistic work has challenged the traditional view of sperm …
Published in International Journal of Animal Biotechnology and Applications · Vol. 12, Issue 1, 2026 Read article
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Animal Cloning using Genetic Engineering and Applications
Abstract: Extracellular pronuclear DNA microinjection in zygotes, injection of genetically modified embryonic stem cells into blastocysts, are all examples of genetic engineering procedures used to create transgenic animal models. Enhancing animal production and productivity, improving animal disease resistance, and biomedical applications are all part of this technique. Cloning is the process of creating animals with the same genetic makeup as the donor nucleus. Somatic cell nuclear transfer has been the most …
Published in International Journal of Animal Biotechnology and Applications · Vol. 8, Issue 1, 2022 · pp. 5–8 Read article