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By: Vishal J. Mistry, Jayesh Rupareliya, and Mansi Gandhi.
Assistant Professor, Department of Biotechnology, Faculty of Science, Monark University, Ahmedabad, Gujarat, India
HOD, Assistant Professor, Department of Bioscience, Shree P.M. Patel Institute of Bioscience and Biotechnology, Anand, Gujarat, India.
Assistant Professor, Department of Chemistry, Faculty of Science, Monark University, Ahmedabad, Gujarat, India.
The Apiaceae family, including economically vital crops like dill, coriander, and fennel, faces significant productivity constraints due to narrow genetic diversity and slow conventional breeding. This study presents a robust molecular breeding framework integrating RAPD and SSR markers to accelerate genetic improvement. Five diverse germplasm accessions were evaluated across morphological, biochemical, and molecular parameters. Molecular analysis revealed substantial polymorphism, with SSR markers demonstrating high informativeness (average PIC = 0.73). Notably, SSR-2 showed a strong positive association with seed yield (r = 0.82), while SSR-4 linked significantly to essential oil content (r = 0.79). UPGMA clustering and PCoA clearly differentiated genotypes into two major clusters, identifying fennel (A3) as the superior genotype for yield and quality. These results validate that molecular marker–based selection enhances accuracy and shortens the breeding cycle. This integrated approach provides a scalable framework for developing resilient, high-yielding Apiaceae cultivars.
Keywords: Apiaceae, Molecular Markers, Marker-Assisted Selection, Genetic Diversity, Crop Improvement
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