By: Madhuri Sharon, Archana Singh, Anuradha Pandey, and Sudhakar More
Director, Sharon Institute of Nanotechnology (SINT), Jaipur, India.
Associate Professor, B.K. Birla College (Empowered Autonomous status)
Research Scholar, Parishkar College of Global Excellence (Autonomous), Jaipur, India.
General Manager, Bliss GVS Pharma Ltd, Mumbai, India.
Leaves of Ancistrocladus heyneanus were collected from the forest of the Matheran hills of Maharashtra. From these leaves, alkaloids were extracted using dichloromethane, and two new monomeric Naphthyl-iso-quinoline alkaloids, Youndamine A and Ancistrocline were isolated using TLC and HPLC. HPLC/MS and NMR further identified the patient. The present study involves the collection and chemical investigation of the leaves of Ancistrocladus heyneanus, a plant species found in the forested regions of the Matheran Hills, Maharashtra, India. The leaves of this species were harvested for alkaloid extraction, focusing on the rich diversity of naphthyl-isoquinoline compounds characteristic of the Ancistrocladaceae family. The collected leaves were processed using dichloromethane as the extraction solvent, which is effective in isolating organic compounds due to its non-polar properties and compatibility with alkaloid structures. Following the extraction, chromatographic techniques, specifically Thin Layer Chromatography (TLC) and High-Performance Liquid Chromatography (HPLC), were employed to separate and isolate the individual alkaloid components present in the extract. This process led to the discovery of two novel monomeric naphthyl-isoquinoline alkaloids, named Youndamine A and Ancistrocline. The initial separation using TLC allowed for the identification of fractions containing distinct alkaloid bands, while HPLC provided further purification and quantification of these compounds. These techniques proved essential for the isolation of pure samples suitable for subsequent analytical studies. For further identification and structural elucidation of Youndamine A and Ancistrocline, advanced spectroscopic methods were utilized. High-Performance Liquid Chromatography coupled with Mass Spectrometry (HPLC/MS) provided detailed molecular weight information and fragmentation patterns, aiding in the confirmation of the molecular structures of the isolated compounds.
Citation:
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