Topical Application of Grape Seed Oil and Linalool, and Treating the Mulberry Leaves with Aqueous Solution of Drakshsava Before Feeding the Fifth Instars of Silkworm, Bombyx mori (L), for Qualitative Silk Cocoons.

Volume: 11 | Issue: 02 | Year 2025 | Subscription
International Journal of Analytical and Applied Chemistry
Received Date: 06/21/2025
Acceptance Date: 06/27/2025
Published On: 2025-08-26
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By: Vitthalrao B. Khyade, Nikhil Pandit Khanapure, and Louis J. Ignarro

Retired Professor, Department of Zoology, Sharadabai Pawar Mahila Arts, Commerce and Science College, Shardanagar, Tal. Baramati Dist. Pune – 413115 (India).
Professor, Department of Chemistry, Punyashlok Ahilyadevi Holkar Solapur University, Solapur (Maharashtra State, India) PIN– 413 255.
Professor, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, 23-315 CHS, 10833 LeConte Avenue, Los Angeles, CA 90095-1735, USA (269 South Beverly Drive, Unit 288, Beverly Hills, CA 90212)

Abstract

Nutrition quality and health of larval-instars exert influence on silk-yield-quality. Ten microliters of Acetone- solutions of Grape-Seed-Oil (10 ml Grape-Seed-Oil dissolved in 90 ml acetone) and acetone solutions of Linalool (100 ppm) were used separately in present attempt for the topical application to the fifth instar larval stages of silkworm, Bombyx mori (L). Group of larvae fed Mulberry-leaves treated with aqueous solution Drakshasav; group of larvae topically applied with acetone solutions of Grape-Seed-Oil (10 ml Grape-Seed-Oil dissolved in 90 ml acetone) followed by feeding Mulberry leaves treated with aqueous solution Drakshasav were also maintained. Fifth-Instar-Larval-Age (Hours) and Tissue-Somatic-Index of silk glands of Untreated- control-group, Grape-Seed-Oil-treatment; Linalool-treatment; Drakshasav-treatment and Grape-Seed-Oil- treatment acetone followed Drakshasav-treatment were recorded 145.33 ( 13.786), 31.426; 168.73( 13.221), 52.625; 177.46 ( 13.786), 52.728; 162.87(14.572), 52.759 and 168.58(18.789), 53.854 units respectively. Shell Ratio of cocoon spun by fifth instar silkworms of Untreated-control-group, Grape-Seed-Oil-treatment; Linalool-treatment; Drakshasav-treatment and Grape-Seed-Oil-treatment acetone followed Drakshasav- treatment were recorded 19.422; 23.970; 27.989; 28.048 and 28.378 units respectively. Denier scale of silk filament spun by fifth instar silkworms of control group, Grape-Seed-Oil treated group; Linalool treated group; group fed with mulberry leaves treated with aqueous solution of Drakshasav and group treated with Grape-Seed- Oil treated acetone (topical) followed feeding by mulberry leaves treated with aqueous solution of Drakshasav were recorded 3.243; 4.706; 4.793; 4.882 and 4.948 units respectively. The range of improvements of tissue somatic index of silk glands (TSI); Shell Ratio of cocoon and Denier scale of silk filament through treatment was 52.625 to 53.854; 23.970 to 28.378 and 4.706 to 4.948. Efficient use of source of juvenoids like linalool, grape seed oil and drakshasav in desired solvent for treating the silkworm serve to orchestrate the fortification of health through the preventing the infection of microbial pathogens; extension of larval-age, consumption-and- utilization of nutrition for significant yield of silk product.

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How to cite this article: Vitthalrao B. Khyade, Nikhil Pandit Khanapure, and Louis J. Ignarro, Topical Application of Grape Seed Oil and Linalool, and Treating the Mulberry Leaves with Aqueous Solution of Drakshsava Before Feeding the Fifth Instars of Silkworm, Bombyx mori (L), for Qualitative Silk Cocoons.. International Journal of Analytical and Applied Chemistry. 2025; 11(02): -p.

How to cite this URL: Vitthalrao B. Khyade, Nikhil Pandit Khanapure, and Louis J. Ignarro, Topical Application of Grape Seed Oil and Linalool, and Treating the Mulberry Leaves with Aqueous Solution of Drakshsava Before Feeding the Fifth Instars of Silkworm, Bombyx mori (L), for Qualitative Silk Cocoons.. International Journal of Analytical and Applied Chemistry. 2025; 11(02): -p. Available from:https://journalspub.com/publication/ijaac/article=20206

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