By: D.V Surya Prakash, Kirti Mittal, Rayna Salvia Joel, and Arjun Sharma
Plumeria rubra, a tropical flowering plant from the Apocynaceae family, holds notable aesthetic and traditional significance. It is frequently grown in tropical areas as an ornamental shrub or small tree, reaching up to six meters in height. Renowned for its visual appeal, it is especially valued in the creation of leis and other floral arrangements. Although the plant’s sap is toxic, it is recognized for its medicinal and aromatic potential. Additionally, it contains various phytochemicals and demonstrates several biological properties. In this research, the extraction process when further optimized, involved various physicochemical parameters that influenced the yield of flavonoids (quercetin and rutin). These parameters were different solvents and their varying concentration, extraction time, pH, and particle mesh size. The optimal conditions for extracting these flavonoids from this plant leaves were found to be ethanol as the solvent with 100% concentration, 24 hrs extraction time at pH-6, and having a particle size of 72 mesh (210 micron). As a result, the concentration of quercetin and rutin of this plant extract wwere found to be 39.0µg/ml and 30.0µg/ml.
Keywords: Â Plumeria rubra, Quercetin, Rutin, Extraction, Ethanol
Citation:
Refrences:
1. Verma, S. Multipurpose ornamental plant Plumeria rubra Linn. (Apocynaceae). Int. J. Sci. Res. Sci. Eng. Technol. 2016, 2, 646–649.
2. Idrees, S.; Hanif, M.A.; Ayub, M.A.; Jilani, M.I.; Memon, N. Frangipani. In Medicinal Plants of South Asia: Novel Sources of Drug Discovery; Hanif, M.A., Nawaz, H., Khan, M.M., Byrne, H.J., Eds.; Elsevier: Amsterdam, The Netherlands, 2019; pp. 287–300.
3. Bihani, T. Plumeria rubra L.—A review on its ethnopharmacological, morphological, phytochemical, pharmacological and toxicological studies. J.Ethnopharmacol. 2021, 264, 113291.
4. Criley, R.A. Plumeria rubra: An old ornamental, a new crop. Acta Hortic. 2009, 813, 183–190.
5. Shinde, P.R.; Patil, P.S.; Bairagi, V.A. Phytopharmacological review of Plumeria species. Sch. Acad. J. Pharm. 2014, 3, 217–227.
6. Andrade-Cetto, A.; Heinrich, M. Mexican plants with hypoglycaemic effect used in the treatment of diabetes. J. Ethnopharmacol. 2005, 99, 325–348.
7. Jarald, E.E.; Joshi, S.B.; Jain, D.C. Diabetes and herbal medicines. Iran. J. Pharmacol. Ther. 2008, 7, 97–106.
8. Kalita, J.C.; Chakrabarty, A.; Tanti, B. Assessment of antifertility activity of some traditionally used plants by different ethnic communities in three districts of Assam, India. J. Herbal. Med. Toxicol. 2011, 5, 65–72.
9. Verma, C.; Bhatia, S.; Srivastava, S. Traditional medicine of the nicobarese. Indian J. Tradit. Knowl. 2010, 9, 779–785.
10. Duarte, M.d.R.; Larrosa, C.R.R. Morpho-anatomical characters of the leaf and stem of Mandevilla coccinea (Hook. et Arn.) Woodson, Apocynaceae. Braz. J. Pharm. Sci. 2011, 47, 137–144.
11. Carvalho, R.; Pellissari, L.C.O.; Pace, M.R.; Scremin-Dias, E.; Arruda, R.D.O.; Farinaccio, M.A. Leaf morphoanatomy of Araujia and Morrenia (Asclepiadoideae, Apocynaceae): Phylogenetic implications and species key. Bot. J. Linn. Soc. 2017, 183, 280–293.
12. Seenu, Y.; Ravichandran, K.R.; Sivadas, A.; Mayakrishnan, B.; Thangavelu, M. Vegetative anatomy of Tabernaemontana alternifolia L. (Apocynaceae) endemic to southern Western Ghats, India. Acta Biol. Szeged. 2020, 63, 185–193.
13. El-Taher, A.M.; Gendy, A.E.-N.G.E.; Alkahtani, J.; Elshamy, A.I.; Abd-ElGawad, A.M. Taxonomic Implication of Integrated Chemical, Morphological, and Anatomical Attributes of Leaves of Eight Apocynaceae Taxa. Diversity 2020, 12, 334.
14. Boldt, K.M.; Rank, B. Stomata dimorphism in dicotyledonous plants of temperate climate. Feddes Repert. 2010, 121, 167–183.
15. Ramos, S.M.B.; Carvalho, M.A.S.; Possobom, C.C.F.; Almeida, E.F.A.; Nietsche, S. Biology and structure of flowers in Adenium obesum (Forssk.) Roem. & Schult. (Apocynaceae) accessions with notes on the significance of these features for floriculture. Braz. J. Bot. 2022, 45, 689–702.
16. Blažeković, B.; Stabentheiner, E.; Brantner, A.; Vladimir-Knežević, S. A Comparative study on glandular trichomes of Lavandula × intermedia ‘Budrovka’ and L. angustifolia. Phyton-Ann. Rei Bot. 2012, 52, 227–244.
17. Kardono, L.B.; Tsauri, S.; Padmawinata, K.; Pezzuto, J.M.; Kinghorn, A.D. Cytotoxic constituents of the bark of Plumeria rubra collected in Indonesia. J. Nat. Prod. 1990, 53, 1447–1455.
18. Ye, G.; Yang, Y.L.; Xia, G.X.; Fan, M.S.; Huang, C.G. Complete NMR spectral assignments of two new iridoid diastereoisomers from the flowers of Plumeria rubra L. cv. acutifolia. Magn. Reson. Chem. 2008, 46, 1195–1197.
19. Kuigoua, G.M.; Kouam, S.F.; Ngadjui, B.T.; Schulz, B.; Green, I.R.; Choudhary, M.I.; Krohn, K. Minor secondary metabolic products from the stem bark of Plumeria rubra Linn. displaying antimicrobial activities. Planta Med. 2010, 76, 620–625.
20. Tohar, N.; Awang, K.; Mohd, M.A.; Jantan, I. Chemical Composition of Four Plumeria Species Grown on Peninsular Malaysia. J. Essent. Oil Res. 2006, 18, 613–617.
21. Guan Ye, Zhi-Xiong Li, Guang-Xin Xia, Hua Peng, ZhaoLin Sun, Cheng-Gang Huang. A New Iridoid Alkaloid from the Flowers of Plumeria rubra L. cv. Acutifolia Helvetica Chimica Acta. 2009; 92:2700-2706.
22. . Ramproshad S, Afroz T, Mondal B, Khan R, Ahmed S. Screening of Phytochemical and Pharmacological activities of leaves of medicinal plant Plumeria rubra, International journal of research in pharmacy and chemistry. 2012; 4:1001-1007.
23. Ye G1, Yang YL, Xia GX, Fan MS, Huang CG. Complete NMR spectral assignments of two new iridoid diastereoisomers from the flowers of Plumeria rubra L. cv. acutifolia. Magn Reson Chem. 2008; 46:1195-7.