Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance
Vishal J. Mistry, Jayesh Rupareliya, Mansi Gandhi | International Journal of Plant Biotechnology | Vol 12, Issue 02 | ISSN: 2456-0162
Abstract
The Apiaceae family, including economically vital crops like dill, coriander, and fennel, faces significant productivity constraints due to narrow genetic diversity and slow conventional breeding. This study presents a robust molecular breeding framework integrating RAPD and SSR markers to accelerate genetic improvement. Five diverse germplasm accessions were evaluated across morphological, biochemical, and molecular parameters. Molecular analysis revealed substantial polymorphism, with SSR markers demonstrating high informativeness (average PIC = 0.73). Notably, SSR-2 showed a strong positive association with seed yield (r = 0.82), while SSR-4 linked significantly to essential oil content (r = 0.79). UPGMA clustering and PCoA clearly differentiated genotypes into two major clusters, identifying fennel (A3) as the superior genotype for yield and quality. These results validate that molecular marker–based selection enhances accuracy and shortens the breeding cycle. This integrated approach provides a scalable framework for developing resilient, high-yielding Apiaceae cultivars.
1. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990;18(22):6531–6535. 2. Baghizadeh A, Karimi MS, Pourseyedi S. Genetic diversity assessment of Iranian green cumin genotypes by RAPD molecular markers. Int J Agron Plant Prod. 2013;4(3):472–479. 3. Zhang L, Li Y, Zhao X. Genetic diversity and population structure analysis in carrot (Daucus carota L.) using SSR markers. Sci Hortic. 2021;279:109902. 4. Wang XJ, Scossa F, Fernie AR. The evolution, domestication and breeding of Apiaceae crops. Plant Biotechnol J. 2022;20(3):451–467. 5. Singh A, Negi PS. Biotechnological application of health-promising bioactive compounds. In: Biotechnological intervention in production of bioactive compounds: biosynthesis, characterization and applications. Cham (CH): Springer Nature Switzerland; 2025. p. 73–94. 6. Choudhary S, Sharma R, Meena RS. Development and characterization of microsatellite markers in coriander (Coriandrum sativum L.) and their application in genetic diversity analysis. Int J Curr Microbiol Appl Sci. 2017;6(8):2550–2560. 7. Paran I, Michelmore RW. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet. 1993;85(8):985–993. 8. Palumbo F, Galla G, Barcaccia G. Development of SSR markers and genome-wide analysis in fennel (Foeniculum vulgare Mill.). Mol Breed. 2018;38(7):88. 9. Kumar S. Survey sequencing and in-silico development and validation of genomic SSR markers in Indian dill seed. J Seed Spices Relat Crops. 2020;10(1):12–18. 10. Sinhasane SR, Shinde US, Benke AP. Understanding of genetic variables for growth and yield traits of Dill (Anethum graveolens L.). Genet Resour Crop Evol. 2022;69(7):2575–2584. 11. Mistry VJ, Parmar RV. Assessment of genetic variation in Indian dill (Anethum graveolens) germplasm using SSR and RAPD molecular markers. South East Eur J Public Health. 2025;25(S2):2820–2832. Available from: https://seejph.com/index.php/seejph/article/view/3204. 12. Kadoglidou K, Cook CM, Boutsika A, Sarrou E. Evaluation of a Dill (Anethum graveolens L.) gene bank germplasm collection using multivariate analysis of morphological traits, molecular genotyping and chemical composition. PeerJ. 2023;11:e15043.
How to cite this article
APA
Mistry, V. J., Rupareliya, J., & Gandhi, M. (2026). Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance . International Journal of Plant Biotechnology, 12(02).
MLA
Mistry, Vishal J., et al. “Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance .” International Journal of Plant Biotechnology, vol. 12, no. 02, 2026.
Chicago
Vishal J. Mistry, Jayesh Rupareliya, and Mansi Gandhi. “Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance .” International Journal of Plant Biotechnology 12, no. 02 (2026).
Vancouver
Mistry VJ, Rupareliya J, Gandhi M. Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance . International Journal of Plant Biotechnology. 2026;12(02).
BibTeX
@article{MistryVJ2026,
author = {Vishal J. Mistry and Jayesh Rupareliya and Mansi Gandhi},
title = {Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance },
journal = {International Journal of Plant Biotechnology},
year = {2026},
volume = {12},
number = {02},
issn = {2456-0162},
url = {https://journalspub.com/publication/ijpb/article=25924}
}
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Vishal J. Mistry, Jayesh Rupareliya, Mansi Gandhi | International Journal of Plant Biotechnology | Vol 12, Issue 02 | ISSN: 2456-0162
Abstract
The Apiaceae family, including economically vital crops like dill, coriander, and fennel, faces significant productivity constraints due to narrow genetic diversity and slow conventional breeding. This study presents a robust molecular breeding framework integrating RAPD and SSR markers to accelerate genetic improvement. Five diverse germplasm accessions were evaluated across morphological, biochemical, and molecular parameters. Molecular analysis revealed substantial polymorphism, with SSR markers demonstrating high informativeness (average PIC = 0.73). Notably, SSR-2 showed a strong positive association with seed yield (r = 0.82), while SSR-4 linked significantly to essential oil content (r = 0.79). UPGMA clustering and PCoA clearly differentiated genotypes into two major clusters, identifying fennel (A3) as the superior genotype for yield and quality. These results validate that molecular marker–based selection enhances accuracy and shortens the breeding cycle. This integrated approach provides a scalable framework for developing resilient, high-yielding Apiaceae cultivars.
1. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990;18(22):6531–6535. 2. Baghizadeh A, Karimi MS, Pourseyedi S. Genetic diversity assessment of Iranian green cumin genotypes by RAPD molecular markers. Int J Agron Plant Prod. 2013;4(3):472–479. 3. Zhang L, Li Y, Zhao X. Genetic diversity and population structure analysis in carrot (Daucus carota L.) using SSR markers. Sci Hortic. 2021;279:109902. 4. Wang XJ, Scossa F, Fernie AR. The evolution, domestication and breeding of Apiaceae crops. Plant Biotechnol J. 2022;20(3):451–467. 5. Singh A, Negi PS. Biotechnological application of health-promising bioactive compounds. In: Biotechnological intervention in production of bioactive compounds: biosynthesis, characterization and applications. Cham (CH): Springer Nature Switzerland; 2025. p. 73–94. 6. Choudhary S, Sharma R, Meena RS. Development and characterization of microsatellite markers in coriander (Coriandrum sativum L.) and their application in genetic diversity analysis. Int J Curr Microbiol Appl Sci. 2017;6(8):2550–2560. 7. Paran I, Michelmore RW. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet. 1993;85(8):985–993. 8. Palumbo F, Galla G, Barcaccia G. Development of SSR markers and genome-wide analysis in fennel (Foeniculum vulgare Mill.). Mol Breed. 2018;38(7):88. 9. Kumar S. Survey sequencing and in-silico development and validation of genomic SSR markers in Indian dill seed. J Seed Spices Relat Crops. 2020;10(1):12–18. 10. Sinhasane SR, Shinde US, Benke AP. Understanding of genetic variables for growth and yield traits of Dill (Anethum graveolens L.). Genet Resour Crop Evol. 2022;69(7):2575–2584. 11. Mistry VJ, Parmar RV. Assessment of genetic variation in Indian dill (Anethum graveolens) germplasm using SSR and RAPD molecular markers. South East Eur J Public Health. 2025;25(S2):2820–2832. Available from: https://seejph.com/index.php/seejph/article/view/3204. 12. Kadoglidou K, Cook CM, Boutsika A, Sarrou E. Evaluation of a Dill (Anethum graveolens L.) gene bank germplasm collection using multivariate analysis of morphological traits, molecular genotyping and chemical composition. PeerJ. 2023;11:e15043.
How to cite this article
APA
Mistry, V. J., Rupareliya, J., & Gandhi, M. (2026). Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance . International Journal of Plant Biotechnology, 12(02).
MLA
Mistry, Vishal J., et al. “Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance .” International Journal of Plant Biotechnology, vol. 12, no. 02, 2026.
Chicago
Vishal J. Mistry, Jayesh Rupareliya, and Mansi Gandhi. “Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance .” International Journal of Plant Biotechnology 12, no. 02 (2026).
Vancouver
Mistry VJ, Rupareliya J, Gandhi M. Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance . International Journal of Plant Biotechnology. 2026;12(02).
BibTeX
@article{MistryVJ2026,
author = {Vishal J. Mistry and Jayesh Rupareliya and Mansi Gandhi},
title = {Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance },
journal = {International Journal of Plant Biotechnology},
year = {2026},
volume = {12},
number = {02},
issn = {2456-0162},
url = {https://journalspub.com/publication/ijpb/article=25924}
}
Vishal J. Mistry, Jayesh Rupareliya, Mansi Gandhi | International Journal of Plant Biotechnology | Vol 12, Issue 02 | ISSN: 2456-0162
Abstract
The Apiaceae family, including economically vital crops like dill, coriander, and fennel, faces significant productivity constraints due to narrow genetic diversity and slow conventional breeding. This study presents a robust molecular breeding framework integrating RAPD and SSR markers to accelerate genetic improvement. Five diverse germplasm accessions were evaluated across morphological, biochemical, and molecular parameters. Molecular analysis revealed substantial polymorphism, with SSR markers demonstrating high informativeness (average PIC = 0.73). Notably, SSR-2 showed a strong positive association with seed yield (r = 0.82), while SSR-4 linked significantly to essential oil content (r = 0.79). UPGMA clustering and PCoA clearly differentiated genotypes into two major clusters, identifying fennel (A3) as the superior genotype for yield and quality. These results validate that molecular marker–based selection enhances accuracy and shortens the breeding cycle. This integrated approach provides a scalable framework for developing resilient, high-yielding Apiaceae cultivars.
1. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990;18(22):6531–6535. 2. Baghizadeh A, Karimi MS, Pourseyedi S. Genetic diversity assessment of Iranian green cumin genotypes by RAPD molecular markers. Int J Agron Plant Prod. 2013;4(3):472–479. 3. Zhang L, Li Y, Zhao X. Genetic diversity and population structure analysis in carrot (Daucus carota L.) using SSR markers. Sci Hortic. 2021;279:109902. 4. Wang XJ, Scossa F, Fernie AR. The evolution, domestication and breeding of Apiaceae crops. Plant Biotechnol J. 2022;20(3):451–467. 5. Singh A, Negi PS. Biotechnological application of health-promising bioactive compounds. In: Biotechnological intervention in production of bioactive compounds: biosynthesis, characterization and applications. Cham (CH): Springer Nature Switzerland; 2025. p. 73–94. 6. Choudhary S, Sharma R, Meena RS. Development and characterization of microsatellite markers in coriander (Coriandrum sativum L.) and their application in genetic diversity analysis. Int J Curr Microbiol Appl Sci. 2017;6(8):2550–2560. 7. Paran I, Michelmore RW. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet. 1993;85(8):985–993. 8. Palumbo F, Galla G, Barcaccia G. Development of SSR markers and genome-wide analysis in fennel (Foeniculum vulgare Mill.). Mol Breed. 2018;38(7):88. 9. Kumar S. Survey sequencing and in-silico development and validation of genomic SSR markers in Indian dill seed. J Seed Spices Relat Crops. 2020;10(1):12–18. 10. Sinhasane SR, Shinde US, Benke AP. Understanding of genetic variables for growth and yield traits of Dill (Anethum graveolens L.). Genet Resour Crop Evol. 2022;69(7):2575–2584. 11. Mistry VJ, Parmar RV. Assessment of genetic variation in Indian dill (Anethum graveolens) germplasm using SSR and RAPD molecular markers. South East Eur J Public Health. 2025;25(S2):2820–2832. Available from: https://seejph.com/index.php/seejph/article/view/3204. 12. Kadoglidou K, Cook CM, Boutsika A, Sarrou E. Evaluation of a Dill (Anethum graveolens L.) gene bank germplasm collection using multivariate analysis of morphological traits, molecular genotyping and chemical composition. PeerJ. 2023;11:e15043.
How to cite this article
APA
Mistry, V. J., Rupareliya, J., & Gandhi, M. (2026). Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance . International Journal of Plant Biotechnology, 12(02).
MLA
Mistry, Vishal J., et al. “Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance .” International Journal of Plant Biotechnology, vol. 12, no. 02, 2026.
Chicago
Vishal J. Mistry, Jayesh Rupareliya, and Mansi Gandhi. “Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance .” International Journal of Plant Biotechnology 12, no. 02 (2026).
Vancouver
Mistry VJ, Rupareliya J, Gandhi M. Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance . International Journal of Plant Biotechnology. 2026;12(02).
BibTeX
@article{MistryVJ2026,
author = {Vishal J. Mistry and Jayesh Rupareliya and Mansi Gandhi},
title = {Modern Molecular Breeding Approaches in Apiaceae for Enhanced Agronomic Performance },
journal = {International Journal of Plant Biotechnology},
year = {2026},
volume = {12},
number = {02},
issn = {2456-0162},
url = {https://journalspub.com/publication/ijpb/article=25924}
}
