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2000 articles for “methods” (ranking capped at the first 2,000 matches — narrow the search to see the rest)
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Synthetic Seed Technology and Its Applications: A Review
Abstract: Synthetic seed technology is a novel approach in plant tissue culture, as it joins the benefits of clonal propagation with those of seed proliferation. Production of synthetic seeds has opened new era in in vitro plant propagation technology, because it offers many useful advantages on a commercial scale for the propagation of a variety of plants. Synthetic seed technology is useful in multiplication and conservation of elite plant species, which …
Published in International Journal of Plant Biotechnology · Vol. 3, Issue 1, 2017 · pp. 11–16 Read article
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Vesicular–Arbuscular Mycorrhiza Association Alleviates Antioxidant Activity of Bacopa monnieri
Abstract: Methanol extract of Bacopa leaves and ascorbic acid showed a significant scavenging of p < 0.05. The study was conducted to evaluate the effect of vesicular–arbuscular mycorrhiza treatment on antioxidant activity of Bacopa monnieri. Mycorrhiza spores were added to the plants at different time intervals and the plants were categorized as T1, T2, T3 and T4 according to treatments. T1 plants are control, while T2, T3 and T4 plants received …
Published in International Journal of Plant Biotechnology · Vol. 4, Issue 1, 2018 · pp. 24–28 Read article
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Prediction of Functional Parameters of Vegetable Stem Solid Waste Degradation Using Taylor’s Variable Model
Abstract: Mathematical model was developed to monitor, predict and simulate the functional parameters of vegetable stem solid waste degradation using Taylor’s variable model. Data obtained from the experimental research work was transformed and resolved using Laplace transformation upon the degradation of vegetable stem solid waste. The specific rate of vegetable stem solid waste, maximum specific rate and the disassociation constant rate were evaluated in terms Michaeli’s Menten and Linear method using …
Published in International Journal of Plant Biotechnology · Vol. 4, Issue 2, 2018 · pp. 30–36 Read article
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Review on: Marker Assisted Selection in Common Bean Breeding for Disease Resistance
Abstract: Common bean (Phaseolus vulgaris L) is the most important food legume consumed worldwide and an important source of human dietary protein, calories, vitamins and minerals necessary for a healthy community. Marker-assisted selection is one of the breeding methods, which involves selection of plants carrying genomic regions that are involved in the expression of traits of interest through molecular markers. The method (MAS) becomes possible for traits governed by major genes …
Published in International Journal of Plant Biotechnology · Vol. 6, Issue 1, 2020 · pp. 23–34 Read article
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To Screen for Citrus Tristeza Virus & Potato Viruses in Stems and Leaves of Plant Samples
Abstract: For centuries, viruses have been known to cause four diseases in almost every plant species and inflict devastating economic losses every year. With the emergence of new infectious diseases via outbreaks, rapid detection and accurate identification with the means of high throughput technology becomes all the more important. The development of numerous diagnostic methods such as ELISA and Lateral Flow Assays which work on the principle of antigen antibody interaction …
Published in International Journal of Plant Biotechnology · Vol. 8, Issue 2, 2022 · pp. 31–51 Read article
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Protein Determination and Soluble Amino Acids Prediction in Various Solvents Extracted Solutions of Indigenous Red Lentil and Moong Pulse through Direct UV- Visible Spectrophotometer Technique: A Comparative Study
Abstract: In this comparative study, direct protein determination have been done for indigenous Red lentil (Lens culinaris) and Moong pulse (Vigna radiata) (obtained from Muzaffarpur, Bihar) by UV-Visible spectroscopy in three different solvents viz. double distilled water (ddH2O), ethyl alcohol (C2H5OH), and methyl alcohol (CH3OH). A few amino acids in solutions have also been predicted (not characterized) based on their peaks observed in UV-region by comparing them with reported range of …
Published in International Journal of Plant Biotechnology · Vol. 9, Issue 2, 2023 · pp. 42–48 Read article
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Clinical Evaluation of Mycobacterium Tuberculosis Culture and Multiplex PCR for the Characterization of Multiple Drug Resistance Tuberculosis in Dehradun, Uttarakhand, India
Abstract: Multiple drug resistance (MDR) tuberculosis timely diagnose is of utmost clinical relevance and needs to be diagnose at initial stages for the proper treatment. The current study was done to detect the multiple genes for MDR tuberculosis (TB) in clinical isolates by molecular tools. 186 clinical samples were considered out and subjected for AFB smear preparation, Nested PCR (IS6110) for Mycobacterium tuberculosis complex detection and MDR TB PCR targeting rpoB, …
Published in International Journal of Animal Biotechnology and Applications · Vol. 3, Issue 2, 2017 · pp. 26–31 Read article
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Virosomes: Understanding the Modern Concepts and Latest Applications
Abstract: A virosome is a cutting-edge hybrid drug delivery method that combines the benefits of viral and non-viral vectors. A virosome has been shown to transport a variety of physiologically active substances, including nucleic acids, peptides, proteins, and tiny chemical compounds, according to research. Surface changes of virosomes may be used to enable targeted medication delivery utilising virosome-based devices. There are presently a variety of virosome-based preventive and therapeutic medicines on …
Published in International Journal of Animal Biotechnology and Applications · Vol. 7, Issue 1, 2021 · pp. 20–31 Read article
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The fundamentals and implications of Animal Tissue Culture
Abstract: A foundation for studying regulation, proliferation, and differentiation as well as a tool for genetic manipulation, animal cell culture technology has today become indispensable in the field of life sciences applications. For it to be completed properly, specialised technical abilities are needed. The fundamental methods of animal cell culture are covered in this review. A foundation for studying regulation, proliferation, and differentiation as well as a tool for genetic manipulation, …
Published in International Journal of Animal Biotechnology and Applications · Vol. 8, Issue 2, 2022 · pp. 8–12 Read article
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Use of Micro- and Nanobubble in Waste Water Treatment Technology – Review
Abstract: ABSTRACT The environmental nanotechnology is a technical discipline which research features of natural and man-made nanomaterials, applications, techniques for their characterization, integration processes and transformation into ecosystems. Microbubbles have the capability to change the normal characteristic of water. The microbubbles (MBs) have diameter more than 100 μm, the micro-nanobubbles (MNBs) have diameter between 1 to 100 μm and the nanobubbles (NBs) have diameter less than 1 μm within the fluid …
Published in International Journal of Industrial Biotechnology and Biomaterials · Vol. 4, Issue 1, 2018 · pp. 5–10 Read article
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High-Performance Thin-Layer Chirography Detection of Ethambutol
Abstract: Ethambutol belongs to the class of organic compounds known as 1, 2 amino alcohols. Being a medication, it is primarily used to treat tuberculosis. Its route of administration is oral. It is readily absorbed by gastrointestinal tract and 50% excreted unchanged by urine. Capacity of protein binding is 20–30%. It is antitubercular agent i.e. inhibiting the synthesis of spermidine in myobacteria also inhibits the transfer of mycolic acids into cell …
Published in International Journal of Industrial Biotechnology and Biomaterials · Vol. 4, Issue 1, 2018 · pp. 31–33 Read article
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Antimicrobial Activity of Waste Pulp of Citrus sinensis and Citrus limetta
Abstract: An antimicrobial is a substance which kills or inhibits the growth of different types of micro-organisms such as bacteria, fungi or protozoans. Citrus fruit products act as antimicrobial agents against the bacteria and the fungus. Citrus fruits belong to the family Rutaceae. The main objective was to determine the antimicrobial activity of Citrus sinensis waste pulp and to determine the antimicrobial activity of Citrus limetta waste pulp. The citrus pulp …
Published in International Journal of Industrial Biotechnology and Biomaterials · Vol. 4, Issue 1, 2018 · pp. 11–22 Read article
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Bioremediation of Oil-Spilled Sites through Seeding Naturally Adapted Pseudomonas putida
Abstract: In the present study, crude oil degradation was analyzed using isolated bacterial strains. These bacterial strains were isolated from the crude-oil-contaminated soil sample. All the isolates were identified as species of Pseudomonas, in which the Pseudomonas putida P11 and LP1 demonstrated a strong ability to degrade kerosene, gasoline, diesel, engine oil and crude oil. Bioremediation for oil spill cleanup was analyzed by the inoculation of P. putida with the addition …
Published in International Journal of Industrial Biotechnology and Biomaterials · Vol. 4, Issue 1, 2018 · pp. 23–30 Read article
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Effect of Pretreatment of Wheat Bran for the Production of Glucoamylase by Aspergillus niger
Abstract: ABSTRACTWheat bran is a complex food waste which contain different nutritional compound in complex form. Microorganisms are not able to utilize these complex foods. The complex food of wheat bran can be simplified by pretreatment method. In this present study the wheat bran are treated with acid and alkali for hydrolysis of complex food in to simple food and then the production of glucoamylase were carried out by Aspergillus Niger. …
Published in International Journal of Industrial Biotechnology and Biomaterials · Vol. 5, Issue 2, 2019 · pp. 10–14 Read article
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Ice Pigging: An Emerging Technique to Replace CIP
Abstract: Ice pigging is potential method to remove fouling from pipes and narrow space in pipelines having better cleaning efficiency to other conventional method. In India the water has insufficiency for drinking,similarly the not proper availability of water also for processing section in dairy and food region.Sometimes water quality is not suitable for cleaning the processing line of Agri-sector.Wastewater has more predominant in the Agri-sector of India. The ice pigging is …
Published in International Journal of Industrial Biotechnology and Biomaterials · Vol. 7, Issue 2, 2021 · pp. 10–15 Read article
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Revolutionizing Healthcare: Cutting-Edge Biosensors Paving the Way for 21st Century Point-of-Care Diagnostics
Abstract: Point-of-care (POC) diagnostics stand as a transformative paradigm in healthcare, promising rapid testing directly at patient care sites. Biosensors, amalgamating biological recognition elements with physico-chemical transducers, emerge as pivotal tools in crafting rapid and sensitive POC diagnostic devices. Recent strides in nanotechnology, microfluidics, and flexible electronics have engendered a wave of innovative biosensor designs and detection methodologies. This paper provides an extensive review of the latest advancements in biosensors tailored …
Published in International Journal of Industrial Biotechnology and Biomaterials · Vol. 9, Issue 2, 2023 · pp. 12–19 Read article
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A Comparative Study on Antioxidant Activities in Selected Citrus Fruits and Herbal Spices
Abstract: The compound which can suppress the free radicals generated in our body is known as antioxidant. Antioxidants are must for defense system of the body. Various types of foods taken by the body play an important role in body protection. Scientific evidence suggests that antioxidants reduce the risk for chronic diseases including cancer and heart disease. Primary sources of naturally occurring antioxidants are whole grains, fruits and vegetables. Plant sourced …
Published in International Journal of Agrochemistry · Vol. 2, Issue 1, 2016 · pp. 17–21 Read article
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MONITORING AND MANAGEMENT OF INSECT PESTS OF MAIZE IN KATHMANDU VALLEY
Abstract: Monitoring study was carried out through survey in Kathmandu valley (Ramkot -5 Kathmandu district, Katunje -5, Bhaktapur district and Bungmati -25 Lalitpur district). Both qualitative and quantitative research approach was use with an objective to find the insect pests of maize in Kathmandu valley. Survey information was collected from 100 households randomly; 33 households from Kathmandu and Lalitpur districts and 34 households from Bhaktapur district during mid - June 2017 …
Published in International Journal of Agrochemistry · Vol. 5, Issue 1, 2019 · pp. 8–15 Read article
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Off Seasonal Tomato Production Practices in Nepal
Abstract: Solanaceous crop, tomato, a good source of minerals, vitamins and organic acids, is used as protective food and grown all over the world as annual plant. In Nepal, farmers are growing it mainly as offseason crop to earn good value even though risk is higher and are facing different problems. Different methods such as exploiting agro climatic diversity, generation of micro-climate, adaptation of grafted plants etc are in use. Use …
Published in International Journal of Agrochemistry · Vol. 5, Issue 2, 2019 · pp. 13–18 Read article
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Farmer’s Perception and Need of Awareness About IT
Abstract: The main purpose of the study was to understand the extent of awareness in farmers about the use of IT. As we know, In the 21st century various technologies are invented. Modern agricultural methods are followed by farmers to some extent but most farmers are still unaware of these technologies. Lack of awareness and lack of education is the problem so this is time to train and spread awareness in …
Published in International Journal of Agrochemistry · Vol. 8, Issue 1, 2022 · pp. 43–49 Read article