Search
21 articles for “protein extraction”
-
Transgenic Animals: Methods and Applications
Abstract: Transgenic animals are those animals whose genetic makeup is altered by ingesting foreign genes into the genes of that animal in order to produce enhanced product. The process of inserting the gene of the desire to express that gene in the animal and then in its offspring by using recombinant DNA technology is termed as transgenesis. With this advancement it has become easier to use the process of transgenesis for …
Published in International Journal of Animal Biotechnology and Applications · Vol. 7, Issue 2, 2021 · pp. 1–4 Read article
-
Protein Determination and Soluble Amino Acids Prediction in Various Solvents Extracted Solutions of Indigenous Red Lentil and Moong Pulse through Direct UV- Visible Spectrophotometer Technique: A Comparative Study
Abstract: In this comparative study, direct protein determination have been done for indigenous Red lentil (Lens culinaris) and Moong pulse (Vigna radiata) (obtained from Muzaffarpur, Bihar) by UV-Visible spectroscopy in three different solvents viz. double distilled water (ddH2O), ethyl alcohol (C2H5OH), and methyl alcohol (CH3OH). A few amino acids in solutions have also been predicted (not characterized) based on their peaks observed in UV-region by comparing them with reported range of …
Published in International Journal of Plant Biotechnology · Vol. 9, Issue 2, 2023 · pp. 42–48 Read article
-
Response Surface Methodology to Optimize Deproteinization of Green Crab Shells (Carcinus maenas)
Abstract: The crab shells are used as a feedstock for the extraction of either chitin or chitosan and other bioactive materials such as dyes and proteins. The optimum conditions are required for a process development for an effective separation of chitin. The most important parameters for deproteinization of green crab shells have identified the concentration of NaOH, temperature and time of contact. The optimum conditions for deproteinization of Green crab shells …
Published in International Journal of Chemical Engineering and Processing · Vol. 3, Issue 2, 2017 · pp. 30–37 Read article
-
Imminent Bioperspectives of Aromatic Plant Leaf Extracts from Cinnamomum tamala and its Biopesticidal Potential against Tribolium castaneum (Herbst.)
Abstract: present research work concluded that when the C. tamala leaf extract of five various solvents revealed that acetone extract possessed potential biopesticidal efficiency because it contains the cinnamaldehyde and linalool like compounds present in the same extract. Totally eleven compounds are elucidated from the leaf (acetone extract) through a GCMS analysis. It was almost conformed that these two aldehyde and aromatic carboxylic acid groups were presented in the acetone extract …
Published in International Journal of Plant Biotechnology · Vol. 5, Issue 2, 2019 · pp. 31–43 Read article
-
Oil Extraction, Biochemical Analysis, and Antimicrobial Activities for Active Compound Valorization in Yellow Mustard (Sinapis alba L.) Seeds and Production of Microbial Oil Using Saccharomyces cerevisiae
Abstract: *Author for CorrespondenceDowluru SVGK Kaladhar E-mail: [email protected] 1Professor, Department of Microbiology and Bioinformatics, UTD, Atal Bihari Vajpayee University, Bilaspur, Chhattisgarh, India2Student, Department of Microbiology and Bioinformatics, UTD, Atal Bihari Vajpayee University, Bilaspur, Chhattisgarh, India3Student, Department of Biotechnology, DP Vipra College, Bilaspur, Chhattisgarh, India Received Date: June 17, 2026Accepted Date: June 24, 2026Published Date: August 15, 2026 Citation: Dowluru S. V. G. K. Kaladhar, Vinay Kumar, Dowluru V. L. Lolini. Oil …
Published in International Journal of Plant Biotechnology · Vol. 12, Issue 2, 2026 · pp. 55–61 Read article
-
Agro Seed Oil Production through Mechanothermal Extraction: Exploring its Potential for Chemical, Pharmaceutical, and Food Industries
Abstract: The increasing demand for sustainable and environmentally friendly industrial raw materials has intensified research into the production of oils from agro seeds using efficient extraction technologies. This study reviews the mechanothermal extraction of agro-seed oils with emphasis on their potential applications in chemical, pharmaceutical, and food industries. Mango (Mangifera indica) seeds, which are commonly discarded as agricultural waste during fruit processing, contain significant quantities of lipids, proteins, carbohydrates, antioxidants, and …
Published in International Journal of Analytical and Applied Chemistry · Vol. 12, Issue 2, 2026 Read article
-
Eco-Friendly Synthesis of Nanoparticles: Advancing Green Methods Using Plant Extracts
Abstract: Nanotechnology, focusing on materials sized 1-100 nm, has garnered significant interest for its applications in agriculture, pharmaceuticals, and electronic devices. Nanomaterials, due to their unique properties, are pivotal in the development of advanced technologies such as drug delivery systems and nano-devices. Conventional nanoparticle synthesis methods, including sol-gel, chemical precipitation, microwave, and chemical vapor deposition, often involve hazardous chemicals, posing environmental risks. Therefore, there is a crucial demand for environmentally friendly …
Published in International journal of Nanobiotechnology · Vol. 10, Issue 1, 2024 Read article
-
Utilization of leaves of mulberry, Morusalba (L) for the preparation of healthy food, Paratha, the cooked dough
Abstract: Indian subcontinent is famous for the paratha, the flatbread. The present attempt deals with preparation of paratha through utilization of leaves of mulberry, Morusalba (L) and it’s assessment for the health parameters. The ingredients used for paratha are whole meal wheat flour; white wheat flour; salt; dried leaves of mulberry, Morus alba (L); curry leaves; herbs de provence; rapeseed oil and fresh extract of leaves of mulberry, Morus alba (L). …
Published in International Journal of Chemical Engineering and Processing · Vol. 6, Issue 1, 2020 · pp. 27–50 Read article
-
A Conceptual study of Metabolism wsr to metabolism of Carbohydrate,Protein and Fat
Abstract: Energy homeostasis is as critical as other vital process of body.It is produced in body by various chemical reaction that take place in each cell of living organism .Unique feature of living organism by which they extract energy from the environment and use it to carry out activities such as growth,development and reproduction.It is a common belief that Thin people have higher metabolic rate and Fat people have lower metabolic …
Published in International Journal of Nursing Critical Care · Vol. 7, Issue 2, 2021 · pp. 22–29 Read article
-
The effect of wheat straw leachate on growth of oyster mushroom (Pleurotus ostreatus) spawn
Abstract: Wheat straw (Triticum aestivum – Duzi) is known for its growth inhibitor in both field and in vitro experiments. The roots of lettuce and wheat seedlings were particularly inhibited when incubated with leachate of wheat straw. In South Africa, mushroom industry is the only industry that directly converts straw into a protein source for human consumption. Therefore, the objectives of the study was to assess the potential growth inhibition of …
Published in International Journal of Agrochemistry · Vol. 5, Issue 2, 2019 · pp. 19–29 Read article
-
Synthesis, Characterization and Optimization of Essential Oil from Pumpkin Seed
Abstract: The main aim of this research work was extraction, characterization and optimization of essential oil from pumpkin seed, cucurbita pepo variety using ethanol as a solvent. The extraction was carried out by using a Soxhlet, leaching method and oil was separated from solvents by using rotary evaporator. Pumpkin seed was analyzed for proximate analysis and found that moisture content of 5.21 , crude proteins content of 22.50.31%, crude fat content …
Published in International Journal of Chemical Separation Technology · Vol. 7, Issue 1, 2021 · pp. 6–27 Read article
-
Pteridophytes as a Source of Traditional Medicine and Environmental Remediation: A Comprehensive Review
Abstract: The Himalayas, one of the worlds’ most important biodiversity hotspots, contribute substantially to the vegetational resources of the Indian subcontinent, with the Garhwal Himalaya standing out for its rich and diverse flora. Among the plant species in this region, pteridophytes, particularly ferns, hold notable economic and medicinal value. These plants have long been integral to traditional medicine systems, such as Ayurveda, and their therapeutic properties have been passed down through …
Published in International Journal of Plant Biotechnology · Vol. 10, Issue 2, 2024 · pp. 33–36 Read article
-
Role of Bioactive Compounds on Protein Utilization in Ruminants: A Review
Abstract: Insufficient nutrition is the primary drive of low productivity by ruminants. These insufficiencies can be corrected by supplementation with high-density feeds and locally available tree legumes. These tree legumes contain various types of bioactive compounds, which can be defined as secondary plant metabolites (PSM) that are extra nutritional substances having pharmacological or toxicological effects in man and animals. Besides the primary biosynthetic, the secondary metabolites are also produced within the …
Published in International Journal of Animal Biotechnology and Applications · Vol. 4, Issue 1, 2018 · pp. 21–26 Read article
-
Extraction of Xylanases from Media Based on three Agricolous Co-products by An Aqueous Two-phase System
Abstract: Jonesia denitrificans BN13 grows very well on a medium made out of agricultural byproducts like orange peel, esparto grass, and retam; the relative enzyme activity of these three substrates was 0.662, 0.711, and 0.835 U/ml, respectively First time xylanases were effectively extracted utilising aqueous two-phase system (ATPS) that had 3.5% PEG, 14% KH2PO4/K2HPO4, and 9% KI, The inclusion of cells resulted in much improved output from the system, which is …
Published in International Journal of Chemical Synthesis and Chemical Reactions · Vol. 8, Issue 2, 2022 · pp. 7–15 Read article
-
Metals from Plantation to Search Toxicity
Abstract: Ascent of sap by plant tissues absorbs chemical complexes for ingrowth. Trace elemental complexes have been as require by successive growth strategies as per standards. Therefore, heavy elements in soil compositions have adhered other than morphological, which have been either in excess or others under composed variations. Soil content ever has studied to be primary issue to assess the possibilities of polluting elemental containment. Examples are innumerable; however some of …
Published in International Journal of Plant Biotechnology · Vol. 6, Issue 1, 2020 · pp. 1–9 Read article
-
Investigation of 2, 4-D Hepatotoxicity and Lepidium sativum Antitoxicity on Rat Tissue using FT-IR Spectroscopy
Abstract: Pesticides have been proven to induce various toxicities in animals, plants and humans. The current study aimed to apply FTIR spectroscopy for the investigation of the antitoxic effect of the medicinal plant Lepidium sativum (LS) against the pesticide 2, 4-dichlorophenoxyacetic acid (2, 4-D) hepatotoxicity. Twelve male albino Wister rats were divided into four groups (control, protective, curative and LS only) and were treated for 4 weeks. Results indicated that the …
Published in International Journal of Animal Biotechnology and Applications · Vol. 6, Issue 1, 2020 · pp. 17–29 Read article
-
Biosynthesis of silver nanoparticles using aqueous leaf extract of Muntingia calabura Linn.
Abstract: The present study was focused to synthesis silver nanoparticles using the aqueous leaf extract of Muntingia calabura L. as the reducing agent. The formation of silver nanoparticles was confirmed by colour change from light yellow to dark brownish red, UV-Visible Spectroscopy, FT-IR, X-Ray Diffraction Method (XRD), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray microanalysis spectroscopy (EDS). The nanoparticles showed an absorbance at 443nm on UV-Visible spectroscopy. The presence of …
Published in International journal of Nanobiotechnology · Vol. 4, Issue 1, 2018 · pp. 9–15 Read article
-
Seaweed Mediated Biosynthesis of Silver Nanoparticles Using Gracilaria fergusonii J.Ag. Collected From Hare Island, Thoothukudi in the South East Coast of Tamil Nadu, India
Abstract: In the present study silver nanoparticles were synthesized using the aqueous extract of red seaweed Gracilaria fergusonii J.Ag. as the reducing agent. The formation of silver nanoparticles was confirmed by colour change from light yellow to dark brownish red, UV– visible spectroscopy, FT-IR, X-ray diffraction method (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis spectroscopy (EDS). The anoparticles showed an absorbance at 442 nm on UV–visible spectroscopy. The …
Published in International Journal of Plant Biotechnology · Vol. 1, Issue 1-2, 2015 · pp. 50–57 Read article
-
In Vitro Evaluation of Bioremediation Capacity of a Commercial Probiotic, Bacillus clausii, for Chromium (VI) and Lead (II) Toxicity
Abstract: Heavy metals cause disastrous effects on any living organisms. Probiotics have the ability to reduce metal toxicity. Commercial probiotics having bioremediation capacity can use these bacteria as an alternative to conventional remediation methods. The bioaccumulation of heavy metals including chromium (VI) (Cr [VI]) and lead (II) (Pb [II]) causes fatal toxicity in humans. Some naturally occurring bacterial species, such as Bacillus and Pseudomonas, contribute their role in bioremediation of these …
Published in International Journal of Industrial Biotechnology and Biomaterials · Vol. 4, Issue 2, 2018 · pp. 1–16 Read article
-
High-Performance Thin-Layer Chirography Detection of Ethambutol
Abstract: Ethambutol belongs to the class of organic compounds known as 1, 2 amino alcohols. Being a medication, it is primarily used to treat tuberculosis. Its route of administration is oral. It is readily absorbed by gastrointestinal tract and 50% excreted unchanged by urine. Capacity of protein binding is 20–30%. It is antitubercular agent i.e. inhibiting the synthesis of spermidine in myobacteria also inhibits the transfer of mycolic acids into cell …
Published in International Journal of Industrial Biotechnology and Biomaterials · Vol. 4, Issue 1, 2018 · pp. 31–33 Read article