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By: Nisha Rani, Anil Kumar, and Kaushal Sanghi.
Silver nanoparticles (Ag NPs) are widely recognized for their applications in medicine, electronics, and environmental remediation. This study presents a new green synthesis method for Ag NPs using Trapa bispinosa fruit extract as a natural reducing agent and stabilizing agent. Characterization of the synthesized Ag NPs through X-ray diffraction (XRD) confirmed a crystalline structure consistent with the face-centered cubic (fcc) arrangement, with peaks at (111), (200), (220), (311), and (222), indicating metallic silver. Fourier Transform Infrared (FTIR) spectroscopy revealed the presence of functional groups, such as O–H and C–N, which play a key role in stabilizing the nanoparticles. Scanning electron microscopy (SEM) and particle size analysis confirmed the formation of spherical nanoparticles with an average size of 82.93 nm, while transmission electron microscopy (TEM) revealed an average particle size of 13.36 nm. Antimicrobial tests demonstrated inhibition zones of 6.9 ± 0.22, 9.7 ± 0.45, and 12.9 ± 0.33 mm at 100, 150, and 200 µg/ml against Staphylococcus aureus, respectively, while Pseudomonas aeruginosa exhibited no inhibition. This study highlights the potential of Trapa bispinosa fruit extract in Ag NPs synthesis, demonstrating its dual role in nanoparticle production and biomedical applications.
Keywords: Ag NPs, Trapa bispinosa, XRD, SEM, TEM, FTIR
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