By: Shalini Rachel and M. Shailaja Raj
Ever since ethanol has proved to be an alternative to fuel, various sources are explored to enhance its production. Thus far, starch and sugar containing substrates, such as sugarcane and corn are employed for commercial production of ethanol resulting in an imbalance in the industrial and domestic supply of sugars causing an impact on their prices. Therefore, this study investigated the potential of mixed floral wastes as a cheaper alternative for production of ethanol. Yeast isolated from sugarcane juice and identified as Pichia kudriavzevii CY 902 was used for fermentation. Growth and cultural characteristics were optimized for incubation period (48hrs), temperature (37℃), pH (5.5), osmotolerance (20%), and alcohol tolerance (10%). The culture could strongly ferment and assimilate carbohydrates, such as glucose, fructose, and sucrose. Comparative studies showed, Pichia kudriavzevii CY 902 had 59% more invertase activity (308.6 IU/min/ml) and could yield 17.51% (99.6 g/L/g) more amount of bioethanol as compared to Saccharomyces cerevisiae 13122 (82.16 g/L/g) after 96hrs of fermentation at 37°C and pH 5.5. The study thereby demonstrates the appropriateness of Pichia kudriavzevii CY 902 as a potent stress tolerant strain for commercial production of bioethanol.
Keywords: Bioethanol, fermentation, mixed floral wastes, Pichia kuridavzevii CY 902, Saccharomyces cerevisiae
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