First Ever Experimental Evidence of Nanoyeast Fermenting Glucose

Volume: 10 | Issue: 02 | Year 2024 | Subscription
International Journal of Energetic Materials
Received Date: 10/18/2024
Acceptance Date: 10/26/2024
Published On: 2024-12-21
First Page: 1
Last Page: 6

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By: Prakash Vaithyanathan

Science Teacher and Innovator, 50, L. B. Road, Adyar, Chennai, Tamil Nadu, India.

Abstract

In one of the earliest attempts, nanoyeast (Saccharomyces cerevisiae) particles were supported on an exotic and novel support[CD1] , namely, silica gel. The presence of nanoparticles of yeast with size as small as 161 nm was reported. Nanoyeast buds of very small size namely 41 nm were also observed in the HR SEM. Analogous to the nanoparticles in the chemical world, size reduction of microorganisms with commercial relevance like, yeast, will have groundbreaking implications in the realm of fermentation, leading to biofuels, biochemicals and biopharmaceuticals production. A mild sonication (40 kHz) driven impregnation technique was used for the preparation of the nanobiocatalyst. The presence of the nanobuds on yeast nanoparticles supported on silica gel were proved unambiguously using SEM with EDXA analysis. From the SEM, nanoyeast particles with varying sizes (40–1000 nm) well dispersed on the silica gel were observed. This is the world’s first report on the production of nanoyeast on such an inexpensive and widely used material, namely, silica gel. The potential of the biocatalyst is demonstrated to produce bioethanol from glucose (10 wt. %). 1H NMR was used for the qualitative and quantitative analysis of the yeast. In a homemade continuous flow fixed bed flow bioreactor packed with the biocatalyst (nanoyeast/silica gel) glucose was converted to bioethanol. On the 2nd and 3rd day of the fermentation, bioethanol concentration of 0.075 wt. % and 0.12 wt. % were obtained from the glucose. As the reactor is yet to attain the criticality, it is surmised that the yields close to the theoretical values (namely 5.1 wt. % bioethanol from glucose) could be realized. This has been a first successful attempt to demonstrate the sustainability, proliferation and fermentative activity of the nanoyeast (Saccharomyces cerevisiae) supported on silica gel. Thus, a breakthrough in the realm of modulating the size of the microorganisms, in general, and fugal strain (yeast) in particular, to the nanodimensions has been achieved. Moreover, the current process offers a logical explanation to the natural phenomena of fermentation of leftover foods on sea sands via the action of the fungal microorganisms on the sea sands.

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Citation:

How to cite this article: Prakash Vaithyanathan, First Ever Experimental Evidence of Nanoyeast Fermenting Glucose. International Journal of Energetic Materials. 2024; 10(02): 1-6p.

How to cite this URL: Prakash Vaithyanathan, First Ever Experimental Evidence of Nanoyeast Fermenting Glucose. International Journal of Energetic Materials. 2024; 10(02): 1-6p. Available from:https://journalspub.com/publication/uncategorized/article=13483

Refrences:

  1. Pulidindi IN, Gedanken A, Schwarz R, et al. Mild sonication accelerates ethanol production by yeast fermentation, Energy Fuels. 2012;26:2352–6.
  2. Pulidindi IN, Kimchi BB, Gedanken A. Can cellulose be a sustainable feedstock for Bioethanol production? Renew. energy. 2014;71:77–80.
  3. Tzhayik O, Pulidindi IN, Gedanken A. Forming nano spherical cellulose containers, Ind Eng Chem Res. 2014;53:13871–80.
  4. Korzen L, Pulidindi IN, Israel A, et al. Single step production of bioethanol from the seaweed Ulva rigida using sonication, RSC Adv. 2015;5:16223–9.
  5. Tabah B, Pulidindi IN, Gedanken A. A study on fermentation kinetics for accelerated production of bioethanol from glucose, sucrose, and molasses. J Bioprocess Biotech. 2015;5:232.
  6. Korzen L, Pulidindi IN, Israel A, et al. Marine integrated culture of carbohydrate rich Ulva rigida for enhanced production of bioethanol. RSC Adv. 2015;5:59251–6.
  7. Victor A, Pulidindi IN, Gedanken A. Assessment of holocellulose for the production of bioethanol by conserving Pinus radiata cones as renewable feedstock. J Environ manag. 2015;162;215–20.
  8. Tabah B, Pulidindi IN, Chitturi VR, et al. Solar energy driven simultaneous saccharification and fermentation (SSF) of starch to bioethanol for fuel cell applications. Chem Sus Chem. 2015;8: 3497–503.
  9. Kumar VB, Pulidindi IN, Kinel Y, et al. Evaluation of the potential of Chlorella vulgaris for bioethanol production, Energy and Fuels. 2016;30:3161–6.
  10. Tabah B, Pulidindi IN, Chitturi VR, et al. Utilization of solar energy for continuous production of bioethanol for energy applications. RSC Adv., 2016;6:24203–9.
  11. Klein M, Griess O, Pulidindi IN, et al. Bioethanol production from Ficus religiosa J Environ Manag. 2016;177:20–5.
  12. Betina T, Pulidindi IN, Chitturi VR, et al. Solar-energy-driven conversion of biomass to bioethanol: a sustainable approach, J Mat Chem A. 2017;5:15486–506.
  13. Pancholi S, Naghera P, Shah M, Pulidindi IN, Selvaraj S, Vaithyanathan P. Nanoyeast supported on silica gel for the continuous flow bioethanol production. IJAME. 2024;2(1):1–7.
  14. Tabah B, Varvak A, Pulidindi IN, et al. Production of 1,3-propanediol from glycerol via fermentation by Saccharomyces cerevisiae. Green Chem. 2016;18:4657–9.
  15. Vaithyanathan, P. Strategy to accelerate the biopharmaceuticals production using Komogataella phaffii (Pichia pastoris). IJAM. 2024; 2 (2): 1-7.

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